注册 | 登录
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   精选抗体   >   标签抗体   >   HA Tag Antibody   

HA Tag Antibody

Mouse Monoclonal Antibody (Mab)

  • 1 - HA Tag Antibody AM1008A
    Anti-HA Tag Antibody at 1:8000 dilution + 12tag recombinant protein Lysates/proteins at 20ng per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 产品详情
  • 文献引用 : 24
  • 实验流程
  • 背景知识
Product info
  • Applications Legend:
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG2b
Clone Names 12CA5
Calculated MW Da Da
Additional info
Other Names Tag from influenza hemagglutinin protein
Target/Specificity KLH conjugated synthetic peptide encoding HA tag (YPYDVPDYA) was used as antigen.
Dilution WB~~1:8000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified by protein G and Ni-NTA nickel affinity chromatography, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHA Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Colorectal cancer cells require glycogen synthase kinase-3β for sustaining mitosis via translocated promoter region (TPR)-dynein interaction.
Author : Dewi FRP1,Domoto T2,Hazawa M3,4,Kobayashi A1,Douwaki T1,Minamoto T2,Wong RW1,3,4.
Oncotarget. 2018 Jan 30;9(17):13337-13352. doi: 10.18632/oncotarget.24344. eCollection 2018 Mar 2.
Aquaporin-4 Cell-Surface Expression and Turnover Are Regulated by Dystroglycan, Dynamin, and the Extracellular Matrix in Astrocytes.
Author : Tham DK1, Joshi B1, Moukhles H1.
PLoS One. 2016 Oct 27;11(10):e0165439. doi: 10.1371/journal.pone.0165439. eCollection 2016.
Sp1 transcription factor promotes TMEPAI gene expression and contributes to cell proliferation.
Author : Li Y1, Guo A1, Feng Y1, Zhang Y1, Wang J1, Jing L1, Yan Y1, Jing L1, Liu Z1, Ma L1, Diao A2.
Cell Prolif. 2016 Sep 14. doi: 10.1111/cpr.12292. [Epub ahead of print]
DELLA proteins interact with FLC to repress flowering transition.
Author : Li M1,An F1,Li W1,Ma M1,Feng Y1,Zhang X1,Guo H1.
J Integr Plant Biol. 2016 Jul;58(7):642-55. doi: 10.1111/jipb.12451. Epub 2016 Mar 2.
Methods to Investigate the Role of Toll-Like Receptors in Allergic Contact Dermatitis.
Author : Schmidt M1, Goebeler M2, Martin SF3.
Methods Mol Biol. 2016;1390:319-40. doi: 10.1007/978-1-4939-3335-8_20.
Yeast GSK-3 kinase regulates astral microtubule function through phosphorylation of the microtubule-stabilizing kinesin Kip2.
Author : Drechsler H1, Tan AN1, Liakopoulos D2.
J Cell Sci. 2015 Nov 1;128(21):3910-21. doi: 10.1242/jcs.166686. Epub 2015 Sep 22.
Increased acid ceramidase expression depends on upregulation of androgen-dependent deubiquitinases, USP2, in a human prostate cancer cell line, LNCaP.
Author : Mizutani N1, Inoue M1, Omori Y1, Ito H1, Tamiya-Koizumi K1, Takagi A1, Kojima T1, Nakamura M2, Iwaki S3, Nakatochi M4, Suzuki M5, Noza
J Biochem. 2015 Oct;158(4):309-19. doi: 10.1093/jb/mvv039. Epub 2015 Apr 17.
Sequential Elution Interactome Analysis of the Mind Bomb 1 Ubiquitin Ligase Reveals a Novel Role in Dendritic Spine Outgrowth.
Author : Mertz J1, Tan H2, Pagala V2, Bai B1, Chen PC1, Li Y1, Cho JH2, Shaw T3, Wang X2, Peng J4.
Mol Cell Proteomics. 2015 Jul;14(7):1898-910. doi: 10.1074/mcp.M114.045898. Epub 2015 Apr 30.
Maintaining glycogen synthase kinase-3 activity is critical for mTOR kinase inhibitors to inhibit cancer cell growth.
Author : Koo J1, Yue P, Gal AA, Khuri FR, Sun SY.
Cancer Res. 2014 May 1;74(9):2555-68. doi: 10.1158/0008-5472.CAN-13-2946. Epub 2014 Mar 13.
Phospholipase D2 mediates survival signaling through direct regulation of Akt in glioblastoma cells.
Author : Bruntz RC1, Taylor HE, Lindsley CW, Brown HA.
J Biol Chem. 2014 Jan 10;289(2):600-16. doi: 10.1074/jbc.M113.532978. Epub 2013 Nov 20.
The novel Akt inhibitor API-1 induces c-FLIP degradation and synergizes with TRAIL to augment apoptosis independent of Akt inhibition.
Author : Li B1, Ren H, Yue P, Chen M, Khuri FR, Sun SY.
Cancer Prev Res (Phila). 2012 Apr;5(4):612-20. doi: 10.1158/1940-6207.CAPR-11-0548. Epub 2012 Feb 16.
HYPOSENSITIVE TO LIGHT, an alpha/beta fold protein, acts downstream of ELONGATED HYPOCOTYL 5 to regulate seedling de-etiolation.
Author : Sun XD, Ni M.
Mol Plant. 2011 Jan;4(1):116-26. doi: 10.1093/mp/ssq055. Epub 2010 Sep 23.
Traf2- and Nck-interacting kinase is essential for canonical Wnt signaling in Xenopus axis formation.
Author : Satow R1, Shitashige M, Jigami T, Honda K, Ono M, Hirohashi S, Yamada T.
J Biol Chem. 2010 Aug 20;285(34):26289-94. doi: 10.1074/jbc.M109.090597. Epub 2010 Jun 21.
Angiotensin II stimulates thick ascending limb superoxide production via protein kinase C(α)-dependent NADPH oxidase activation.
Author : Herrera M, Silva GB, Garvin JL.
J Biol Chem. 2010 Jul 9;285(28):21323-8. doi: 10.1074/jbc.M110.109157. Epub 2010 May 6.
c-FLIP degradation mediates sensitization of pancreatic cancer cells to TRAIL-induced apoptosis by the histone deacetylase inhibitor LBH589.
Author : Kauh J, Fan S, Xia M, Yue P, Yang L, Khuri FR, Sun SY.
PLoS One. 2010 Apr 28;5(4):e10376. doi: 10.1371/journal.pone.0010376.
FOXO3 modulates endothelial gene expression and function by classical and alternative mechanisms.
Author : Czymai T, Viemann D, Sticht C, Molema G, Goebeler M, Schmidt M.
J Biol Chem. 2010 Apr 2;285(14):10163-78. doi: 10.1074/jbc.M109.056663. Epub 2010 Feb 1.
Blimp1-mediated repression of negative regulators is required for osteoclast differentiation.
Author : Nishikawa K, Nakashima T, Hayashi M, Fukunaga T, Kato S, Kodama T, Takahashi S, Calame K, Takayanagi H.
Proc Natl Acad Sci U S A. 2010 Feb 16;107(7):3117-22. doi: 10.1073/pnas.0912779107. Epub 2010 Jan 28.
Beta-catenin/T-cell factor signaling is activated during lung injury and promotes the survival and migration of alveolar epithelial cells.
Author : Flozak AS, Lam AP, Russell S, Jain M, Peled ON, Sheppard KA, Beri R, Mutlu GM, Budinger GR, Gottardi CJ.
J Biol Chem. 2010 Jan 29;285(5):3157-67. doi: 10.1074/jbc.M109.070326. Epub 2009 Nov 20.
Direct repression of KNOX loci by the ASYMMETRIC LEAVES1 complex of Arabidopsis.
Author : Guo M, Thomas J, Collins G, Timmermans MC.
Plant Cell. 2008 Jan;20(1):48-58. doi: 10.1105/tpc.107.056127. Epub 2008 Jan 18.
CCAAT/enhancer binding protein homologous protein-dependent death receptor 5 induction and ubiquitin/proteasome-mediated cellular FLICE-inhibitory protein down-regulation contribute to enhancement of tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by dimethyl-celecoxib in human non small-cell lung cancer cells.
Author : Chen S, Liu X, Yue P, Schönthal AH, Khuri FR, Sun SY.
Mol Pharmacol. 2007 Nov;72(5):1269-79. Epub 2007 Aug 7.
Neuronal morphogenesis is regulated by the interplay between cyclin-dependent kinase 5 and the ubiquitin ligase mind bomb 1.
Author : Choe EA, Liao L, Zhou JY, Cheng D, Duong DM, Jin P, Tsai LH, Peng J.
J Neurosci. 2007 Aug 29;27(35):9503-12.
Phenethyl isothiocyanate, a cancer chemopreventive constituent of cruciferous vegetables, inhibits cap-dependent translation by regulating the level and phosphorylation of 4E-BP1.
Author : Hu J, Straub J, Xiao D, Singh SV, Yang HS, Sonenberg N, Vatsyayan J.
Cancer Res. 2007 Apr 15;67(8):3569-73.
Genetic regulators of large-scale transcriptional signatures in cancer.
Author : Adler AS, Lin M, Horlings H, Nuyten DS, van de Vijver MJ, Chang HY.
Nat Genet. 2006 Apr;38(4):421-30. Epub 2006 Mar 5.
RACK1 regulates G1/S progression by suppressing Src kinase activity.
Author : Mamidipudi V, Zhang J, Lee KC, Cartwright CA.
Mol Cell Biol. 2004 Aug;24(15):6788-98.


Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-HA monoclonal antibody (Clone 12CA5) provides a simple solution to detect the expression of an HA-tagged protein in cells.


Kolodziej, PA and Young RA. (1991) Methods Enzymol., 194:508-19. Sells MA and Chernoff J. (1995) Gene, 152:187-9.


点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,

如有疑问,联系:0512-88856768 tech-china@abcepta.com.