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UBC9 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - UBC9 Antibody AM1261a
    All lanes : Anti-UBC9 Antibody at 1:1000-1:2000 dilution Lane 1: human heart lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - UBC9 Antibody AM1261a
    Western blot analysis of anti-UBC9 Antibody (Cat. #AM1261a) in Ramos cell line lysates (35μg/lane). UBC9(arrow) was detected using the purified Mab.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P63279
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names 67AT1273.95.90
Calculated MW 18007 Da
Additional info
Gene ID 7329
Other Names SUMO-conjugating enzyme UBC9, 632-, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18, UBE2I, UBC9, UBCE9
Target/Specificity This UBC9 antibody was raised using purified GST-UBC9 fusion protein.
Dilution WB~~1:100~500
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBC9 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE2I
Synonyms UBC9, UBCE9
Function Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4 and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'.
Cellular Location Nucleus. Cytoplasm. Note=Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity).
Tissue Location Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
Research Areas
Differential effects of SUMO1 and SUMO3 on PKR activation and stability.
Author : Maarifi G1,El Asmi F1,Maroui MA1,Dianoux L1,Chelbi-Alix MK2.
Sci Rep. 2018 Jan 19;8(1):1277. doi: 10.1038/s41598-018-19683-6.
29352251
MxA Mediates SUMO-Induced Resistance to Vesicular Stomatitis Virus.
Author : Maarifi G1, Hannoun Z1, Geoffroy MC1, El Asmi F1, Zarrouk K1, Nisole S1, Blondel D2, Chelbi-Alix MK3.
J Virol. 2016 Jun 24;90(14):6598-610. doi: 10.1128/JVI.00722-16. Print 2016 Jul 15.
27170750
TRIM5α is a SUMO substrate.
Author : Dutrieux J1, Portilho DM2, Arhel NJ3, Hazan U4, Nisole S5.
Retrovirology. 2015 Mar 24;12:28. doi: 10.1186/s12977-015-0155-7.
25880753
The SUMOylation of matrix protein M1 modulates the assembly and morphogenesis of influenza A virus.
Author : Wu CY, Jeng KS, Lai MM.
J Virol. 2011 Jul;85(13):6618-28. doi: 10.1128/JVI.02401-10. Epub 2011 Apr 20.
21507966

BACKGROUND

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

REFERENCES

Expression analysis of Ubc9, the single small ubiquitin-like modifier (SUMO) E2 conjugating enzyme, in normal and malignant tissues. Moschos SJ, et al. Hum Pathol, 2010 Sep. PMID 20561671. Ubc9 promotes breast cell invasion and metastasis in a sumoylation-independent manner. Zhu S, et al. Oncogene, 2010 Mar 25. PMID 20023705. Association of SUMO1 and UBC9 genotypes with tumor response in non-small-cell lung cancer treated with irinotecan-based chemotherapy. Han JY, et al. Pharmacogenomics J, 2010 Apr. PMID 19859084. Characterization of papillomavirus E1 helicase mutants defective for interaction with the SUMO-conjugating enzyme Ubc9. Fradet-Turcotte A, et al. Virology, 2009 Dec 20. PMID 19836047. Ubc9 gene polymorphisms and late-onset Alzheimer's disease in the Korean population: a genetic association study. Ahn K, et al. Neurosci Lett, 2009 Nov 20. PMID 19765634.

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