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EZH2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 14 - EZH2 Antibody AM1836A
    AM1836A staining EZH2 in human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 14 - EZH2 Antibody AM1836A
    AM1836A staining EZH2 in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 3 - EZH2 Antibody AM1836A
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling EZH2 with AM1836A at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NA166821) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).
  • 1 - EZH2 Antibody AM1836A
    All lanes : Anti-EZH2 Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 85 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - EZH2 Antibody AM1836A
    Fluorescent image of U251 cell stained with EZH2 Antibody(Cat#AM1836a/SG100830AA).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EZH2 primary antibody (1:25, 1 h at 37℃. For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37℃.Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃.EZH2 immunoreactivity is localized to Nucleus significantly.
  • 14 - EZH2 Antibody AM1836A
    EZH2 Monoclonal Antibody (Cat. #AM1836a) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EZH2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, WB, E
Primary Accession Q15910
Other Accession Q4R381
Reactivity Human
Predicted Monkey
Host Mouse
Clonality Monoclonal
Isotype IgG1,IgK
Clone Names 144CT2.1.1.5
Calculated MW 85363 Da
Additional info
Gene ID 2146
Other Names Histone-lysine N-methyltransferase EZH2, ENX-1, Enhancer of zeste homolog 2, Lysine N-methyltransferase 6, EZH2, KMT6
Target/Specificity This EZH2 antibody is generated from mouse immunized with EZH2 recombinant protein.
Dilution IHC-P~~1:10~50
IF~~1:10~50
WB~~1:2000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEZH2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EZH2 (HGNC:3527)
Synonyms KMT6
Function Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys- 27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2 (PubMed:22323599). Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non- histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.
Cellular Location Nucleus
Tissue Location Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.
Research Areas
Ezh2 regulates adult hippocampal neurogenesis and memory.
Author : Zhang J1, Ji F, Liu Y, Lei X, Li H, Ji G, Yuan Z, Jiao J.
J Neurosci. 2014 Apr 9;34(15):5184-99. doi: 10.1523/JNEUROSCI.4129-13.2014.
24719098

BACKGROUND

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene.

REFERENCES

Long noncoding RNA as modular scaffold of histone modification complexes. Tsai MC, et al. Science, 2010 Aug 6. PMID 20616235. Implications of enhancer of zeste homologue 2 expression in pancreatic ductal adenocarcinoma. Toll AD, et al. Hum Pathol, 2010 Sep. PMID 20573371. Distinctive expression of the polycomb group proteins Bmi1 polycomb ring finger oncogene and enhancer of zeste homolog 2 in nonsmall cell lung cancers and their clinical and clinicopathologic significance. Kikuchi J, et al. Cancer, 2010 Jun 15. PMID 20564407. EZH2 and STAT6 expression profiles are correlated with colorectal cancer stage and prognosis. Wang CG, et al. World J Gastroenterol, 2010 May 21. PMID 20480530. ETS transcription factors control transcription of EZH2 and epigenetic silencing of the tumor suppressor gene Nkx3.1 in prostate cancer. Kunderfranco P, et al. PLoS One, 2010 May 10. PMID 20479932.

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