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MMP2 Antibody (Ascites)

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - MMP2 Antibody (Ascites) AM1844a
    Western blot analysis of MMP2 Antibody (Cat. #AM1844a) in T47D cell line lysates (35μg/lane).MMP2 (arrow) was detected using the purified Mab.(1:200)
  • 14 - MMP2 Antibody (Ascites) AM1844a
    MMP2 antibody (Cat. #AM1844a) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MMP2 antibody for immunohistochemistry. Clinical relevance has not been evaluated.
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  • 文献引用 : 4
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P08253
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b,k
Clone Names 6E3F8
Calculated MW 73882 Da
Additional info
Gene ID 4313
Other Names 72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, TBE-1, PEX, MMP2, CLG4A
Target/Specificity This MMP2 antibody is generated from mouse immunized with MMP2 recombinant protein.
Dilution WB~~1:200~1000
IHC-P~~1:50~100
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMMP2 Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MMP2
Synonyms CLG4A
Function Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro- inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
Cellular Location Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note=Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes
Tissue Location Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
Research Areas
DHRS2 inhibits cell growth and motility in esophageal squamous cell carcinoma.
Author : Zhou Y1,2,Wang L1,Ban X1,Zeng T1,Zhu Y1,Li M1,Guan XY1,3,Li Y1.
Oncogene. 2017 Nov 6. doi: 10.1038/onc.2017.383. [Epub ahead of print]
29106393
Association of MMP-2 and MMP-9 expression with recurrences in primary spontaneous pneumothorax.
Author : Huang YF1,Chiu WC2,Chou SH2,Su YH3,Chen YW1,Chai CY4,Huang CJ5,Huang MY5,Yuan SF6,Lee YC7.
Kaohsiung J Med Sci. 2017 Jan;33(1):17-23. doi: 10.1016/j.kjms.2016.10.007. Epub 2016 Nov 12.
28088269
The CD147/MMP-2 signaling pathway may regulate early stage cardiac remodeling in spontaneously hypertensive rats.
Author : Li B1, Zhou W1, Yang X2, Zhou Y1, Tan Y1, Yuan C1, Song Y3, Chen X4, Zhang W1.
Clin Exp Pharmacol Physiol. 2016 Jul 25. doi: 10.1111/1440-1681.12626. [Epub ahead of print]
27451961
EFEMP1 promotes the migration and invasion of osteosarcoma via MMP-2 with induction by AEG-1 via NF-κB signaling pathway.
Author : Wang Z1, Cao CJ1, Huang LL1, Ke ZF1, Luo CJ1, Lin ZW2, Wang F1, Zhang YQ3, Wang LT1.
Oncotarget. 2015 Jun 10;6(16):14191-208.
25987128

BACKGROUND

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene.

REFERENCES

Role of functional single nucleotide polymorphisms of MMP1, MMP2, and MMP9 in open angle glaucomas. Mossböck G, et al. Mol Vis, 2010 Aug 28. PMID 20808730. Clinical Impact of MMP and TIMP Gene Polymorphisms in Gastric Cancer. Alakus H, et al. World J Surg, 2010 Aug 21. PMID 20730428. A genetic association study of maternal and fetal candidate genes that predispose to preterm prelabor rupture of membranes (PROM). Romero R, et al. Am J Obstet Gynecol, 2010 Jul 29. PMID 20673868. Genetic variants in COL2A1, COL11A2, and IRF6 contribute risk to nonsyndromic cleft palate. Nikopensius T, et al. Birth Defects Res A Clin Mol Teratol, 2010 Jul 29. PMID 20672350. [Expressions of CD147, MMP-2 and MMP-9 in laryngeal carcinoma and clinical significance] Gou XX, et al. Zhonghua Yi Xue Za Zhi, 2010 May 11. PMID 20646600.

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