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GFAP Antibody

Mouse Monoclonal Antibody (Mab)

     
  • 59 - GFAP Antibody AM1870B
    Immunohistochemical analysis of paraffin-embedded human brain tissue using AM1870B performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - GFAP Antibody AM1870B
    All lanes : Anti-GFAP Antibody at 1:4000 dilution Lane 1: human brain lysate Lane 2: human cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - GFAP Antibody AM1870B
    GFAP Antibody (Cat. #AM1870b) western blot analysis in MCF-7 cell line lysates (35μg/lane).This demonstrates the GFAP antibody detected the GFAP protein (arrow).
  • 3 - GFAP Antibody AM1870B
    Confocal immunofluorescent analysis of GFAP Antibody (Cat#AM1870b) with brain tissue followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P-Leica, E
Primary Accession P14136
Other Accession NP_001124491.1
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b,k
Clone Names 183CT3.1.5
Calculated MW 49880 Da
Additional info
Gene ID 2670
Other Names Glial fibrillary acidic protein, GFAP, GFAP
Target/Specificity This GFAP monoclonal antibody is generated from mouse immunized with GFAP recombinant protein.
Dilution IHC-P-Leica~~1:1000
WB~~1:100~500
IF~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGFAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GFAP
Function GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Cellular Location Cytoplasm. Note=Associated with intermediate filaments
Tissue Location Expressed in cells lacking fibronectin.
Research Areas
Reprogramming Postnatal Human Epidermal Keratinocytes Toward Functional Neural Crest Fates.
Author : Bajpai VK1,Kerosuo L2,Tseropoulos G1,Cummings KA3,Wang X1,Lei P1,Liu B4,5,Liu S4,5,Popescu GK3,Bronner ME2,Andreadis ST1,6,7.
Stem Cells. 2017 May;35(5):1402-1415. doi: 10.1002/stem.2583. Epub 2017 Mar 5.
28142205

BACKGROUND

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

REFERENCES

van den Berge, S.A., et al. Aging Cell 9(3):313-326(2010) Martins-de-Souza, D., et al. J Psychiatr Res (2010) In press : Bargagna-Mohan, P., et al. J. Biol. Chem. 285(10):7657-7669(2010) Sultana, R., et al. Antioxid. Redox Signal. 12(3):327-336(2010) Middeldorp, J., et al. PLoS ONE 4 (11), E7663 (2009) :

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