|Application ||WB, E|
|Calculated MW||28362 Da|
|Other Names||Eukaryotic translation initiation factor 4E type 2, eIF-4E type 2, eIF4E type 2, Eukaryotic translation initiation factor 4E homologous protein, Eukaryotic translation initiation factor 4E-like 3, eIF4E-like protein 4E-LP, mRNA cap-binding protein 4EHP, mRNA cap-binding protein type 3, EIF4E2, EIF4EL3|
|Target/Specificity||This EIF4E2 monoclonal antibody is generated from mouse immunized with EIF4E2 recombinant protein.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EIF4E2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation (PubMed:9582349, PubMed:17368478, PubMed:25624349). Acts as a repressor of translation initiation (PubMed:22751931). In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3), suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap (By similarity).|
Provided below are standard protocols that you may find useful for product applications.
EIF4E2 recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Rosettani, P., et al. J. Mol. Biol. 368(3):691-705(2007)
Zuberek, J., et al. RNA 13(5):691-697(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :