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SOX2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
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  • 1 - SOX2 Antibody  AM2048a
    Western blot analysis of lysate from NCCIT cell line, using SOX2 Antibody(Cat. #AM2048a). AM2048a was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20μg.
  • 1 - SOX2 Antibody  AM2048a
    Western blot analysis of lysate from SOX2 protein, using SOX2 Antibody(Cat. #AM2048a). AM2048a was diluted at 1:4000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20μg.
  • 3 - SOX2 Antibody  AM2048a
    Fluorescent image of A549 cell stained with SOX2 Antibody(Cat#AM2048a/SG110310AA).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SOX2 primary antibody (1:25, 1 h at 37℃. For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37℃.Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃.SOX2 immunoreactivity is localized to Nucleus significantly.
  • 1 - SOX2 Antibody  AM2048a
    Western blot analysis of SOX2 Antibody (Cat.#AM2048a) by SOX2 recombinant protein. SOX2(arrow) was detected using the purified Mab.
  • 1 - SOX2 Antibody  AM2048a
    Western blot analysis of SOX2 (arrow) using mouse monoclonal SOX2 antibody (Cat.#AM2048a). 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies)
  • 14 - SOX2 Antibody  AM2048a
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 Antibody (Cat.#AM2048a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - SOX2 Antibody  AM2048a
    Flow cytometric analysis of NCI-H460 cells using SOX2 Monoclonal Antibody (bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.
  • 3 - SOX2 Antibody  AM2048a
    Fluorescent confocal image of SY5Y cells stained with SOX2 antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM2048a SOX2 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Note the highly specific localization of the SOX2 mainly to the nucleus.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IF, WB, IHC-P, FC, E
Primary Accession P48431
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Clone Names 57CT23.3.4
Additional info
Gene ID 6657
Other Names Transcription factor SOX-2, SOX2
Target/Specificity SOX2 recombinant protein is used to produce this monoclonal antibody.
Dilution WB~~1:200~2000
IF~~1:100
IHC-P~~1:50~100
FC~~1:10~50
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSOX2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SOX2
Function Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
Cellular Location Nucleus.
Research Areas
CRISPR/Cas9 targeted deletion of polyglutamine in spinocerebellar ataxia type 3 derived induced pluripotent stem cells.
Author : Ouyang S,Xie Y,Xiong Z,Yang Y,Xian Y,Ou Z,Song B,Chen Y,Xie Y,Li H,Sun X.
Stem Cells Dev. 2018 Apr 17. doi: 10.1089/scd.2017.0209. [Epub ahead of print]
29661116
Physiological genomics identifies genetic modifiers of long QT syndrome type 2 severity.
Author : Chai S1,2,Wan X2,Ramirez-Navarro A2,Tesar PJ3,Kaufman ES2,Ficker E2,George AL Jr.4,Deschênes I1,2.
J Clin Invest. 2018 Feb 12. pii: 94996. doi: 10.1172/JCI94996. [Epub ahead of print]
29431731
Generation of GZKHQi001-A and GZWWTi001-A, two induced pluripotent stem cell lines derived from peripheral blood mononuclear cells of Duchenne muscular dystrophy patients.
Author : Yuhuan X1,Yingjun X1,Yanting X1,Yuchang C1,Bing S1,Shaoying L1,Haoxian L1,Yexing X1,Shuming O1,Zeyu X1,Xiaofang S2.
Stem Cell Res. 2018 Jan 31;28:25-28. doi: 10.1016/j.scr.2018.01.028. [Epub ahead of print]
29414414
Generation of integration-free induced pluripotent stem cells (GZHMUi001-A) by reprogramming peripheral blood mononuclear cells from a 47, XXX syndrome patient.
Author : Chen Y1,Ou Z1,Song B1,Xian Y1,Ouyang S1,Xie Y1,Xue Y1,Sun X2.
Stem Cell Res. 2017 Aug;23:57-60. doi: 10.1016/j.scr.2017.06.002. Epub 2017 Jun 9.
28925367
Brother of the regulator of the imprinted site (BORIS) variant subfamily 6 is a novel target of lung cancer stem-like cell immunotherapy.
Author : Horibe R1,2,Hirohashi Y1,Asano T1,3,Mariya T1,3,Suzuki T4,Takaya A1,Saijo H1,2,Shionoya Y1,2,Kubo T1,Nakatsugawa M1,Kanaseki T1,Tsukahara T1,Watanabe K1,5,Atsuyama E5,Toji S5,Hirano H6,Hasegawa T6,Takahashi H2,Sato N1,Torigoe T1.
PLoS One. 2017 Mar 1;12(3):e0171460. doi: 10.1371/journal.pone.0171460. eCollection 2017.
28248963
Melatonin Inhibits Glioblastoma Stem-like cells through Suppression of EZH2-NOTCH1 Signaling Axis.
Author : Zheng X1,Pang B2,Gu G3,Gao T1,Zhang R1,Pang Q1,Liu Q3.
Int J Biol Sci. 2017 Feb 6;13(2):245-253. doi: 10.7150/ijbs.16818. eCollection 2017.
28255276
ROCK Inhibition Facilitates In Vitro Expansion of Glioblastoma Stem-Like Cells.
Author : Tilson SG1, Haley EM1, Triantafillu UL1, Dozier DA1, Langford CP2, Gillespie GY2, Kim Y1.
PLoS One. 2015 Jul 13;10(7):e0132823. doi: 10.1371/journal.pone.0132823. eCollection 2015.
26167936

BACKGROUND

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT).

REFERENCES

References for protein:
1.Effects of MCF2L2, ADIPOQ and SOX2 genetic polymorphisms on the development of nephropathy in type 1 Diabetes Mellitus. Zhang D, et al. BMC Med Genet, 2010 Jul 28. PMID 20667095.
2.Sox2 protein expression is an independent poor prognostic indicator in stage I lung adenocarcinoma. Sholl LM, et al. Am J Surg Pathol, 2010 Aug. PMID 20631605.
3.Sox2 is translationally activated by eukaryotic initiation factor 4E in human glioma-initiating cells. Ge Y, et al. Biochem Biophys Res Commun, 2010 Jul 9. PMID 20537983.
4.Mutational screening of CHX10, GDF6, OTX2, RAX and SOX2 genes in 50 unrelated microphthalmia-anophthalmia-coloboma (MAC) spectrum cases. Gonzalez-Rodriguez J, et al. Br J Ophthalmol, 2010 Aug. PMID 20494911.
4.Examination of SOX2 in variable ocular conditions identifies a recurrent deletion in microphthalmia and lack of mutations in other phenotypes. Reis LM, et al. Mol Vis, 2010 Apr 28. PMID 20454695.
References for SY5Y (SH-SY5Y; ATCC#CRL-2266): 1. Ross RA, et al. Coordinate morphological and biochemical interconversion of human neuroblastoma cells. J. Natl. Cancer Inst. 71: 741-749, 1983. [PubMed: 6137586]; 2. Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. [PubMed: 29704].
References for MCF7 cell line:
1.Soule, HD; Vazquez J; Long A; Albert S; Brennan M. (1973). "A human cell line from a pleural effusion derived from a breast carcinoma". Journal of the National Cancer Institute 51 (5): 1409–1416. [PMID 4357757].
2.Levenson, AS; Jordan VC. (1997). "MCF-7: the first hormone-responsive breast cancer cell line". Cancer Research 57 (15): 3071–3078. [PMID 9242427].
3.Lacroix, M; Leclercq G. (2004). "Relevance of breast cancer cell lines as models for breast tumours: an update". Breast Research and Treatment 83 (3): 249–289.[PMID 14758095].

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