|Application ||WB, E|
|Reactivity||Human, Mouse, Rat|
|Other Names||Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, GAD-65, Glutamate decarboxylase 65 kDa isoform, GAD2, GAD65|
|Target/Specificity||This GAD2 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 445-473 amino acids from human GAD2.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GAD2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the production of GABA.|
|Cellular Location||Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters|
Provided below are standard protocols that you may find useful for product applications.
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :