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ALB Antibody

Mouse Monoclonal Antibody (Mab)

     
  • 1 - ALB Antibody AM2069b
    ALB Antibody (Cat. #AM2069b) western blot analysis in T47D cell line lysates (35μg/lane).This demonstrates the ALB antibody detected the ALB protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P02768
Other Accession A2V9Z4, NP_000468.1
Reactivity Human
Predicted Monkey
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Clone Names 500CT2.1.4
Calculated MW 69367 Da
Additional info
Gene ID 213
Other Names Serum albumin, ALB
Target/Specificity This ALB antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 540-569 amino acids from human ALB.
Dilution WB~~1:100~500
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALB Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALB
Function Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Cellular Location Secreted.
Tissue Location Plasma.
Research Areas

BACKGROUND

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

REFERENCES

Schelleman, H., et al. Br J Clin Pharmacol 70(3):393-399(2010)
Minchiotti, L., et al. Hum. Mutat. 29(8):1007-1016(2008)
Rikova, K., et al. Cell 131(6):1190-1203(2007)
Sugio, S., et al. Protein Eng. 12(6):439-446(1999)
Sakamoto, Y., et al. Biochim. Biophys. Acta 1252(2):209-216(1995)

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