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>   首页   >   产品   >   一抗   >   信号转导   >   BLNK Antibody (Ascites)   

BLNK Antibody (Ascites)

Mouse Monoclonal Antibody (Mab)

     
  • 1 - BLNK Antibody (Ascites) AM2071a
    BLNK Antibody (Cat. #AM2071a) western blot analysis in CEM cell line lysates (35μg/lane).This demonstrates the BLNK antibody detected the BLNK protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q8WV28
Other Accession NP_001107566.1
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgM
Clone Names 520CT6.1.1
Calculated MW 50466 Da
Additional info
Gene ID 29760
Other Names B-cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, SLP-65, BLNK, BASH, SLP65
Target/Specificity This BLNK antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 150-178 amino acids from human BLNK.
Dilution WB~~1:500~8000
Format Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBLNK Antibody (Ascites) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BLNK
Synonyms BASH, SLP65
Function Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR- mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.
Cellular Location Cytoplasm. Cell membrane. Note=BCR activation results in the translocation to membrane fraction
Tissue Location Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon
Research Areas

BACKGROUND

This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

REFERENCES

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Oellerich, T., et al. Mol. Cell Proteomics 8(7):1738-1750(2009)
Imamura, Y., et al. J. Biol. Chem. 284(15):9804-9813(2009)
Li, H., et al. PLoS ONE 4 (7), E6410 (2009) :

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