|Application ||WB, IHC-P, E|
|Calculated MW||59903 Da|
|Other Names||Protein arginine N-methyltransferase 3, 211-, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, PRMT3, HRMT1L3|
|Target/Specificity||This PRMT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142-173 amino acids from the N-terminal region of human PRMT3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PRMT3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.|
Provided below are standard protocols that you may find useful for product applications.
Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.
Tang, J., et al., J. Biol. Chem. 273(27):16935-16945 (1998).