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CHPT1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - CHPT1 Antibody (N-term) AP10186a
    CHPT1 Antibody (N-term) (Cat. #AP10186a) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 1 - CHPT1 Antibody (N-term) AP10186a
    CHPT1 Antibody (N-term) (Cat. #AP10186a) western blot analysis in Hela,HepG2,K562,MDA-MB453 cell line lysates (35ug/lane).This demonstrates the CHPT1 antibody detected the CHPT1 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession Q8WUD6
Other Accession Q8C025, Q1LZE6, Q8WUD6-2, NP_064629.2
Reactivity Human
Predicted Bovine, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 45097 Da
Additional info
Gene ID 56994
Other Names Cholinephosphotransferase 1, hCPT1, AAPT1-like protein, Diacylglycerol cholinephosphotransferase 1, CHPT1, CPT1
Target/Specificity This CHPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-33 amino acids from the N-terminal region of human CHPT1.
Dilution FC~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCHPT1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CHPT1
Synonyms CPT1
Function Catalyzes phosphatidylcholine biosynthesis from CDP- choline. It thereby plays a central role in the formation and maintenance of vesicular membranes.
Cellular Location Golgi apparatus membrane; Multi-pass membrane protein
Tissue Location Highly expressed in testis, colon, small intestine, heart, prostate and spleen. Also detected in kidney, skeletal muscle, pancreas, leukocytes, ovary and thymus. Weakly expressed in the brain, placenta and lung. Overexpressed in cancerous breast epithelial cell lines
Research Areas

REFERENCES

Akech, J., et al. Biochem. Biophys. Res. Commun. 333(1):35-41(2005)
Ghosh, A., et al. Biochem. Biophys. Res. Commun. 297(4):1043-1048(2002)
Henneberry, A.L., et al. J. Biol. Chem. 275(38):29808-29815(2000)

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