|Application ||IHC-P, FC, IF, WB, E|
|Calculated MW||29097 Da|
|Other Names||Transcription factor A, mitochondrial, mtTFA, Mitochondrial transcription factor 1, MtTF1, Transcription factor 6, TCF-6, Transcription factor 6-like 2, TFAM, TCF6, TCF6L2|
|Target/Specificity||This TFAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-246 amino acids from the C-terminal region of human TFAM.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TFAM Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (PubMed:29445193). Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA.|
|Cellular Location||Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid|
Provided below are standard protocols that you may find useful for product applications.
This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells.
Wang, W., et al. Nucleic Acids Res. (2010) In press :
Corneveaux, J.J., et al. Hum. Mol. Genet. 19(16):3295-3301(2010)
Akhmetov, I.I., et al. Fiziol Cheloveka 36(2):121-125(2010)
Shulman, J.M., et al. PLoS ONE 5 (6), E11244 (2010) :
Laumet, G., et al. J. Alzheimers Dis. 20(4):1181-1188(2010)