注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   癌症   >   HIF1AN Antibody (C-term)   

HIF1AN Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - HIF1AN Antibody (C-term) AP1029b
    Western blot analysis of anti-HIF1AN (C-term) Pab in A375 and mouse brain cell line lysate. HIF1AN (C-term)(arrow) was detected using the purified Pab.
  • 产品详情
  • 文献引用 : 1
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9NWT6
Other Accession Q8BLR9, P59723
Reactivity Human, Mouse
Predicted Zebrafish
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40285 Da
Additional info
Gene ID 55662
Other Names Hypoxia-inducible factor 1-alpha inhibitor, 11411n4, Factor inhibiting HIF-1, FIH-1, Hypoxia-inducible factor asparagine hydroxylase, HIF1AN, FIH1
Target/Specificity This HIF1AN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-316 amino acids from the C-terminal region of human HIF1AN.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIF1AN Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HIF1AN
Synonyms FIH1
Function Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300- interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
Cellular Location Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Mainly cytoplasmic localization, but interaction with NOTCH1 results in nuclear localization and interaction with ABPA3 results in perinuclear localization in macrophages
Research Areas
miR-148a-3p Mediates Notch Signaling to Promote the Differentiation and M1 Activation of Macrophages.
Author : Huang F1,2,Zhao JL1,Wang L1,Gao CC1,Liang SQ1,An DJ1,Bai J1,Chen Y3,Han H1,Qin HY1.
Front Immunol. 2017 Oct 16;8:1327. doi: 10.3389/fimmu.2017.01327. eCollection 2017.
29085372

BACKGROUND

FIH1Encoded protein (factor inhibiting HIF-1)is a co-repressor that interacts with hypoxia-inducible factor 1(HIF-1) alpha and the von Hippel-Lindau tumor suppressor protein to mediate repression of HIF-1 transcriptional activity.

FeedBack

终于等到您。ABCEPTA(百远生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-88856768 tech-china@abcepta.com.


我要评价