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DNMT1 Antibody (S1105)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - DNMT1 Antibody (S1105) AP1032b
    Western blot analysis of DNMT1 Antibody (S1105) (Cat.# AP1032b) in MDA-MB435 cell line lysates (35ug/lane). DNMT1 (arrow) was detected using the purified Pab.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P26358
Other Accession Q9Z330, P13864, NP_001370
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 183165 Da
Additional info
Gene ID 1786
Other Names DNA (cytosine-5)-methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI, MHsaI, MCMT, DNMT1, AIM, CXXC9, DNMT
Target/Specificity This DNMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1083-1112 amino acids from human DNMT1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDNMT1 Antibody (S1105) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNMT1
Synonyms AIM, CXXC9, DNMT
Function Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). Promotes tumor growth (PubMed:24623306).
Cellular Location Nucleus.
Tissue Location Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
Research Areas
Role of epigenetic regulation on the induction of apoptosis in Jurkat leukemia cells by white grape pomace rich in phenolic compounds.
Author : León-González AJ1,Jara-Palacios MJ2,Abbas M1,Heredia FJ2,Schini-Kerth VB1.
Food Funct. 2017 Oct 4. doi: 10.1039/c7fo00263g. [Epub ahead of print]
28976514
Dose-response analysis of epigenetic, metabolic, and apical endpoints after short-term exposure to experimental hepatotoxicants.
Author : Miousse IR1,Murphy LA2,Lin H3,Schisler MR4,Sun J5,Chalbot MG6,Sura R7,Johnson K8,LeBaron MJ9,Kavouras IG10,Schnackenberg LK11,Beger RD12,Rasoulpour RJ13,Koturbash I14.
Food Chem Toxicol. 2017 May 8. pii: S0278-6915(17)30241-7. doi: 10.1016/j.fct.2017.05.013. [Epub ahead of print]
28495587
Reg3g Promotes Pancreatic Carcinogenesis in a Murine Model of Chronic Pancreatitis.
Author : Yin G1, Du J, Cao H, Liu X, Xu Q, Xiang M.
Dig Dis Sci. 2015 Jul 17. [Epub ahead of print]
26182900
OxLDL up-regulates microRNA-29b, leading to epigenetic modifications of MMP-2/MMP-9 genes: a novel mechanism for cardiovascular diseases.
Author : Chen KC1, Wang YS, Hu CY, Chang WC, Liao YC, Dai CY, Juo SH.
FASEB J. 2011 May;25(5):1718-28. doi: 10.1096/fj.10-174904. Epub 2011 Jan 25.
21266537

BACKGROUND

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.

REFERENCES

Peterson, E.J., et al., Cancer Res. 63(20):6579-6582 (2003).
Leu, Y.W., et al., Cancer Res. 63(19):6110-6115 (2003).
Saito, Y., et al., Int. J. Cancer 105(4):527-532 (2003).
Siedlecki, P., et al., Biochem. Biophys. Res. Commun. 306(2):558-563 (2003).
Macaluso, M., et al., Oncogene 22(23):3511-3517 (2003).

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