- 文献引用 : 10
|Application ||WB, E|
|Other Accession||Q1LZ53, O88508, Q4W5Z4|
|Reactivity||Human, Mouse, Rat|
|Predicted||Mouse, Rat, Chicken|
|Other Names||DNA (cytosine-5)-methyltransferase 3A, Dnmt3a, DNA methyltransferase HsaIIIA, DNA MTase HsaIIIA, MHsaIIIA, DNMT3A|
|Target/Specificity||This Dnmt3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 457-486 amino acids from human Dnmt3a.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Dnmt3a Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity.|
|Cellular Location||Nucleus. Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin|
|Tissue Location||Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.|
Author : Bing Y1, Zhu S1, Yu G1, Li T1, Liu W1, Li C1, Wang Y1, Qi H1, Guo T1, Yuan Y1, He Y1, Liu Z2, Liu Q3.
J Biol Chem. 2014 Nov 21;289(47):32639-55. doi: 10.1074/jbc.M114.589689. Epub 2014 Sep 30.
Author : Siddiqi S, Terry M, Matushansky I.
PLoS One. 2012;7(3):e33711. doi: 10.1371/journal.pone.0033711. Epub 2012 Mar 16.
Author : Koturbash I, Zemp F, Kolb B, Kovalchuk O.
Mutat Res. 2011 Jun 17;722(2):114-8. doi: 10.1016/j.mrgentox.2010.05.007. Epub 2010 May 15.
Author : Chen KC1, Wang YS, Hu CY, Chang WC, Liao YC, Dai CY, Juo SH.
FASEB J. 2011 May;25(5):1718-28. doi: 10.1096/fj.10-174904. Epub 2011 Jan 25.
Author : Yamagata Y, Asada H, Tamura I, Lee L, Maekawa R, Taniguchi K, Taketani T, Matsuoka A, Tamura H, Sugino N.
Hum Reprod. 2009 May;24(5):1126-32. doi: 10.1093/humrep/dep015. Epub 2009 Feb 6.
Author : Koturbash I, Boyko A, Rodriguez-Juarez R, McDonald RJ, Tryndyak VP, Kovalchuk I, Pogribny IP, Kovalchuk O.
Carcinogenesis. 2007 Aug;28(8):1831-8. Epub 2007 Mar 7.
Author : Ishii T, Kohu K, Yamada S, Ishidoya S, Kanto S, Fuji H, Moriya T, Satake M, Arai Y.
Tohoku J Exp Med. 2007 Jun;212(2):177-90.
Author : Tryndyak VP, Muskhelishvili L, Kovalchuk O, Rodriguez-Juarez R, Montgomery B, Churchwell MI, Ross SA, Beland FA, Pogribny IP.
Carcinogenesis. 2006 Aug;27(8):1713-20. Epub 2006 Apr 22.
Author : Koturbash I, Rugo RE, Hendricks CA, Loree J, Thibault B, Kutanzi K, Pogribny I, Yanch JC, Engelward BP, Kovalchuk O.
Oncogene. 2006 Jul 20;25(31):4267-75. Epub 2006 Mar 13.
Author : Pogribny I, Koturbash I, Tryndyak V, Hudson D, Stevenson SM, Sedelnikova O, Bonner W, Kovalchuk O.
Mol Cancer Res. 2005 Oct;3(10):553-61.
Provided below are standard protocols that you may find useful for product applications.
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3a is a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
Xie, S., et al., Gene 236(1):87-95 (1999). Robertson, K.D., et al., Nucleic Acids Res. 27(11):2291-2298 (1999).