- 文献引用 : 14
|Application ||IHC-P, WB, E|
|Other Names||DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, MHsaIIIB, DNMT3B|
|Target/Specificity||This Dnmt3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-417 amino acids from human Dnmt3b.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Dnmt3b Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co- repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells (PubMed:27153398).|
|Tissue Location||Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate|
Author : Fu S1,Sun L1,Zhang X1,Shi H1,Xu K1,Xiao Y2,Ye W3.
Exp Eye Res. 2017 Sep 9. pii: S0014-4835(17)30104-5. doi: 10.1016/j.exer.2017.09.004. [Epub ahead of print]
Author : Koturbash I1,Jadavji NM2,Kutanzi K1,Rodriguez-Juarez R1,Kogosov D1,Metz GAS2,3,Kovalchuk O1,3.
Environ Epigenet. 2017 Jan 31;2(4):dvw025. doi: 10.1093/eep/dvw025. eCollection 2016 Dec.
Author : Li C1, Ebert PJ, Li QJ.
J Biol Chem. 2013 Jun 28;288(26):19127-39. doi: 10.1074/jbc.M113.453357. Epub 2013 May 16.
Author : Chen KC1, Wang YS, Hu CY, Chang WC, Liao YC, Dai CY, Juo SH.
FASEB J. 2011 May;25(5):1718-28. doi: 10.1096/fj.10-174904. Epub 2011 Jan 25.
Author : Sohn BH, Park IY, Lee JJ, Yang SJ, Jang YJ, Park KC, Kim DJ, Lee DC, Sohn HA, Kim TW, Yoo HS, Choi JY, Bae YS, Yeom YI.
Gastroenterology. 2010 May;138(5):1898-908. doi: 10.1053/j.gastro.2009.12.044. Epub 2009 Dec 29.
Author : Lu R, Markowetz F, Unwin RD, Leek JT, Airoldi EM, MacArthur BD, Lachmann A, Rozov R, Ma'ayan A, Boyer LA, Troyanskaya OG, Whetton AD, Lemischka IR.
Nature. 2009 Nov 19;462(7271):358-62. doi: 10.1038/nature08575.
Author : Yamagata Y, Asada H, Tamura I, Lee L, Maekawa R, Taniguchi K, Taketani T, Matsuoka A, Tamura H, Sugino N.
Hum Reprod. 2009 May;24(5):1126-32. doi: 10.1093/humrep/dep015. Epub 2009 Feb 6.
Author : Fabbri M, Garzon R, Cimmino A, Liu Z, Zanesi N, Callegari E, Liu S, Alder H, Costinean S, Fernandez-Cymering C, Volinia S, Guler G, Morrison CD, Chan KK, Marcucci G, Calin GA, Huebner K, Croce CM.
Proc Natl Acad Sci U S A. 2007 Oct 2;104(40):15805-10. Epub 2007 Sep 21.
Author : Koturbash I, Boyko A, Rodriguez-Juarez R, McDonald RJ, Tryndyak VP, Kovalchuk I, Pogribny IP, Kovalchuk O.
Carcinogenesis. 2007 Aug;28(8):1831-8. Epub 2007 Mar 7.
Author : Park IY, Sohn BH, Yu E, Suh DJ, Chung YH, Lee JH, Surzycki SJ, Lee YI.
Gastroenterology. 2007 Apr;132(4):1476-94. Epub 2007 Jan 25.
Author : Tryndyak VP, Muskhelishvili L, Kovalchuk O, Rodriguez-Juarez R, Montgomery B, Churchwell MI, Ross SA, Beland FA, Pogribny IP.
Carcinogenesis. 2006 Aug;27(8):1713-20. Epub 2006 Apr 22.
Author : Koturbash I, Rugo RE, Hendricks CA, Loree J, Thibault B, Kutanzi K, Pogribny I, Yanch JC, Engelward BP, Kovalchuk O.
Oncogene. 2006 Jul 20;25(31):4267-75. Epub 2006 Mar 13.
Author : Pogribny I, Koturbash I, Tryndyak V, Hudson D, Stevenson SM, Sedelnikova O, Bonner W, Kovalchuk O.
Mol Cancer Res. 2005 Oct;3(10):553-61.
Author : Brueckner B, Garcia Boy R, Siedlecki P, Musch T, Kliem HC, Zielenkiewicz P, Suhai S, Wiessler M, Lyko F.
Cancer Res. 2005 Jul 15;65(14):6305-11.
Provided below are standard protocols that you may find useful for product applications.
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3b is a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF)syndrome.
Okano, M., et al., Cell 99(3):247-257 (1999). Yin, B., et al., Zhongguo Yi Xue Ke Xue Yuan Xue Bao 21(6):431-438 (1999). Okano, M., et al., Nat. Genet. 19(3):219-220 (1998).