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AP3S1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - AP3S1 Antibody (N-term) AP10499a
    AP3S1 Antibody (N-term) (Cat. #AP10499a) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the AP3S1 antibody detected the AP3S1 protein (arrow).
  • 1 - AP3S1 Antibody (N-term) AP10499a
    AP3S1 Antibody (N-term) (Cat. #AP10499a) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the AP3S1 antibody detected the AP3S1 protein (arrow).
  • 14 - AP3S1 Antibody (N-term) AP10499a
    AP3S1 antibody (N-term) (Cat. #AP10499a) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AP3S1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q92572
Other Accession Q9DCR2, Q2YDH6, NP_001275.1
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 21732 Da
Additional info
Gene ID 1176
Other Names AP-3 complex subunit sigma-1, AP-3 complex subunit sigma-3A, Adaptor-related protein complex 3 subunit sigma-1, Clathrin-associated/assembly/adaptor protein, small 3, Sigma-3A-adaptin, Sigma3A-adaptin, Sigma-adaptin 3a, AP3S1, CLAPS3
Target/Specificity This AP3S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human AP3S1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAP3S1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AP3S1
Synonyms CLAPS3
Function Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.
Cellular Location Golgi apparatus. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side Note=Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex
Tissue Location Present in all adult tissues examined.
Research Areas

BACKGROUND

Part of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

REFERENCES

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Zhou, J.B., et al. Med. Sci. Monit. 16 (6), BR179-BR183 (2010) :
Lefrancois, S., et al. Dev. Cell 7(4):619-625(2004)
Salazar, G., et al. Mol. Biol. Cell 15(2):575-587(2004)
Nie, Z., et al. Dev. Cell 5(3):513-521(2003)

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