|Application ||WB, IHC-P, E|
|Calculated MW||28766 Da|
|Other Names||Phosphoglycerate mutase 2, BPG-dependent PGAM 2, Muscle-specific phosphoglycerate mutase, Phosphoglycerate mutase isozyme M, PGAM-M, PGAM2, PGAMM|
|Target/Specificity||This PGAM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-41 amino acids from the N-terminal region of human PGAM2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PGAM2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Interconversion of 3- and 2-phosphoglycerate with 2,3- bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 18.104.22.168 (synthase), but with a reduced activity.|
|Tissue Location||Expressed in the heart and muscle. Not found in the liver and brain.|
Provided below are standard protocols that you may find useful for product applications.
Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). PGAM2 encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X.
Hadjigeorgiou, G.M., et al. Neuromuscul. Disord. 9 (6-7), 399-402 (1999) :
Tsujino, S., et al. Am. J. Hum. Genet. 52(3):472-477(1993)
Castella-Escola, J., et al. Gene 91(2):225-232(1990)
Castella-Escola, J., et al. Hum. Genet. 84(2):210-212(1990)
Tsujino, S., et al. J. Biol. Chem. 264(26):15334-15337(1989)