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>   首页   >   产品   >   一抗   >   信号转导   >   ENT1(Slc29a1) Antibody (S254)   

ENT1(Slc29a1) Antibody (S254)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ENT1(Slc29a1) Antibody (S254) AP1086d
    All lanes : Anti-ENT1(Slc29a1)-Ps254 at 1:500-1000 dilution Lane 1: 293 whole cell lysate Lane 2: MDA-MB-453 whole cell lysate Lane 3: Mouse lung tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q99808
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 50219 Da
Additional info
Gene ID 2030
Other Names Equilibrative nucleoside transporter 1, Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter, Equilibrative NBMPR-sensitive nucleoside transporter, Nucleoside transporter, es-type, Solute carrier family 29 member 1, SLC29A1, ENT1
Target/Specificity This ENT1(Slc29a1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-260 amino acids from human ENT1(Slc29a1).
Dilution WB~~1:500-1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsENT1(Slc29a1) Antibody (S254) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLC29A1
Synonyms ENT1
Function Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).
Cellular Location Basolateral cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein Note=Predominantly localized in the basolateral membrane in polarized MDCK cells
Tissue Location Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen
Research Areas

BACKGROUND

ENT1 is a member of the equilibrative nucleoside transporter family. It is a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies.

REFERENCES

Bone,D.B.,Am. J. Physiol. Heart Circ. Physiol. 293 (6), H3325-H3332 (2007)
Damaraju,V.L., Am. J. Physiol. Renal Physiol. 293 (1), F200-F211 (2007)
Abdulla,P.,Nucleosides Nucleotides Nucleic Acids 26 (1), 99-110 (2007)
Sundaram,M., J. Biol. Chem. 276 (48), 45270-45275 (2001)

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