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RSBN1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

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AP11537a staining RSBN1 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-RSBN1 Antibody (N-term) at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: human liver lysate Lane 4: MCF-7 whole cell lysate Lane 5: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 90 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	Q5VWQ0
Other Accession	NP_060834.2
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	90072 Da
Antigen Region	51-78 aa

Additional Information
Gene ID	54665
Other Names	Round spermatid basic protein 1, RSBN1
Target/Specificity	This RSBN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-78 amino acids from the N-terminal region of human RSBN1.
Dilution	WB~~1:2000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	RSBN1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	RSBN1
Function	Histone demethylase that specifically demethylates dimethylated 'Lys-20' of histone H4 (H4K20me2), thereby modulating chromosome architecture.
Cellular Location	Nucleus.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

REFERENCES

Rose, J. Phd, et al. Mol. Med. (2010) In press :
Prahalad, S., et al. Arthritis Rheum. 60(7):2124-2130(2009)
Cooper, J.D., et al. Nat. Genet. 40(12):1399-1401(2008)
Raychaudhuri, S., et al. Nat. Genet. 40(10):1216-1223(2008)
Smyth, D.J., et al. Diabetes 57(6):1730-1737(2008)

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¥ 1,250.00

Cat# AP11537a

Size:

50 µl

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Price:

¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

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RSBN1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

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