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IGKV A18 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - IGKV A18  Antibody (C-term) AP11694b
    IGKV A18 Antibody (C-term) (Cat. #AP11694b) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the IGKVA18 antibody detected the IGKVA18 protein (arrow).
  • 4 - IGKV A18  Antibody (C-term) AP11694b
    IGKV A18 Antibody (C-term) (Cat. #AP11694b) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession A2NJV5
Other Accession P01631, P06310, P06309, P01617
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 13085 Da
Additional info
Other Names IGKV A18
Target/Specificity This IGKV A18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-107 amino acids from the C-terminal region of human IGKV A18.
Dilution WB~~1:1000
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIGKV A18 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IGKV2-29 {ECO:0000303|PubMed:11549845, ECO:0000303|Ref.4}
Function V region of the variable domain of immunoglobulin light chains that participates in the antigen recognition (PubMed:24600447). Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:20176268, PubMed:17576170).
Cellular Location Secreted. Cell membrane
Research Areas
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