|Application ||IHC-P, WB, IF, FC, E|
|Other Accession||P41135, P20067, NP_851998.1, NP_002156.2|
|Other Names||DNA-binding protein inhibitor ID-1, Class B basic helix-loop-helix protein 24, bHLHb24, Inhibitor of DNA binding 1, Inhibitor of differentiation 1, ID1, BHLHB24, ID|
|Target/Specificity||This ID1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-93 amino acids from the Central region of human ID1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ID1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).|
|Cellular Location||Cytoplasm. Nucleus.|
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a helix-loop-helix (HLH) protein that can form heterodimers with members of the basic HLH family of transcription factors. The encoded protein has no DNA binding activity and therefore can inhibit the DNA binding and transcriptional activation ability of basic HLH proteins with which it interacts. This protein may play a role in cell growth, senescence, and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Qian, T., et al. Oncogene 29(43):5818-5827(2010)
Hamajima, Y., et al. Cell Prolif. 43(5):457-463(2010)
Yang, J., et al. Circ. Res. 107(2):252-262(2010)
Phi, J.H., et al. J Neurosurg Pediatr 5(6):608-614(2010)
Geng, H., et al. Cancer Res. 70(8):3239-3248(2010)