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TUBB2B Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 59 - TUBB2B Antibody (N-term) AP11940a
    Immunohistochemical analysis of paraffin-embedded Human brain tissue using AP11940A performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:2000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - TUBB2B Antibody (N-term) AP11940a
    All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse cerebellum tissue lysate Lane 7: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, WB, E
Primary Accession Q9BVA1
Other Accession P02554, P13602, P32882, Q3KRE8, Q9CWF2, Q6B856, P85108, Q7TMM9, Q4R5B3, Q13885, P09203, NP_821080.1
Reactivity Human, Mouse, Rat
Predicted Mouse, Rat, Monkey, Pig, Chicken, Xenopus, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 347733
Other Names Tubulin beta-2B chain, TUBB2B
Target/Specificity This TUBB2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human TUBB2B.
Dilution IHC-P-Leica~~1:2000
WB~~1:4000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTUBB2B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TUBB2B
Function Tubulin is the major constituent of microtubules (PubMed:23001566, PubMed:28013290, PubMed:26732629). It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). Plays a critical role in proper axon guidance in both central and peripheral axon tracts (PubMed:23001566). Implicated in neuronal migration (PubMed:19465910).
Cellular Location Cytoplasm, cytoskeleton
Tissue Location High expression in brain.
Research Areas

BACKGROUND

The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. This gene is highly similar to TUBB2A and TUBB2C. Defects in this gene are a cause of asymmetric polymicrogyria. [provided by RefSeq].

REFERENCES

Xu, W., et al. Mol. Cancer Ther. 8(12):3318-3330(2009)
Jaglin, X.H., et al. Nat. Genet. 41(6):746-752(2009)
Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)
Cucchiarelli, V., et al. Cell Motil. Cytoskeleton 65(8):675-685(2008)
Lamesch, P., et al. Genomics 89(3):307-315(2007)

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