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NEU2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 59 - NEU2 Antibody (N-term) AP12189A
    Immunohistochemical analysis of AP12189a on paraffin-embedded human kidney tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.
  • 59 - NEU2 Antibody (N-term) AP12189A
    Immunohistochemical analysis of AP12189a on paraffin-embedded human skeletal muscle tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.
  • 1 - NEU2 Antibody (N-term) AP12189A
    Anti-NEU2 Antibody (N-term) at 1:2000 dilution + Human heart tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - NEU2 Antibody (N-term) AP12189A
    Anti-NEU2 Antibody (N-term) at 1:1000 dilution + human fetal liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - NEU2 Antibody (N-term) AP12189A
    NEU2 Antibody (N-term) (Cat. #AP12189a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NEU2 antibody detected the NEU2 protein (arrow).
  • 3 - NEU2 Antibody (N-term) AP12189A
    Confocal immunofluorescent analysis of NEU2 Antibody (N-term)(Cat#AP12189a) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
  • 4 - NEU2 Antibody (N-term) AP12189A
    NEU2 Antibody (N-term) (Cat. #AP12189a) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, WB, IF, FC, E
Primary Accession Q9Y3R4
Other Accession NP_005374.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42254 Da
Additional info
Gene ID 4759
Other Names Sialidase-2, Cytosolic sialidase, N-acetyl-alpha-neuraminidase 2, NEU2
Target/Specificity This NEU2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-50 amino acids from the N-terminal region of human NEU2.
Dilution IHC-P-Leica~~1:250
WB~~1:1000
IF~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNEU2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NEU2
Function Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins, oligosaccharides and gangliosides.
Cellular Location Cytoplasm.
Tissue Location Expressed in skeletal muscle, fetal liver and embryonic carcinoma cell line NT2-D1
Research Areas

BACKGROUND

This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. [provided by RefSeq].

REFERENCES

Stoppani, E., et al. Cell Biol. Int. 33(9):1020-1025(2009)
Li, C.Y., et al. Cell Res. 17(4):357-362(2007)
Chavas, L.M., et al. J. Biol. Chem. 280(1):469-475(2005)
Seyrantepe, V., et al. J. Biol. Chem. 279(35):37021-37029(2004)
Tringali, C., et al. J. Biol. Chem. 279(5):3169-3179(2004)

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