- 文献引用 : 4
|Application ||WB, E|
|Other Accession||P33436, P50757, P33434, Q90611, NP_004521.1, NP_001121363.1|
|Predicted||Chicken, Mouse, Rabbit, Rat|
|Calculated MW||73882 Da|
|Other Names||72 kDa type IV collagenase, 72 kDa gelatinase, Gelatinase A, Matrix metalloproteinase-2, MMP-2, TBE-1, PEX, MMP2, CLG4A|
|Target/Specificity||This MMP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-331 amino acids from the Central region of human MMP2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MMP2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues in association with MMP14. Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro- inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.|
|Cellular Location||Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note=Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes|
|Tissue Location||Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.|
Author : Wang Z#1,Li R#1,He Y1,Huang S1.
Cancer Cell Int. 2018 Mar 27;18:48. doi: 10.1186/s12935-018-0543-x. eCollection 2018.
Author : Chen J1,2,Liu H3,Gao P2,Hui Y2,Yang Z2,Zhang X3,Xu P4,Tian F5,Fan T2.
Tumour Biol. 2017 May;39(5):1010428317708267. doi: 10.1177/1010428317708267.
Author : Dai Y1, Duan H2, Duan C3, Zhou R1, He Y1, Tu Q1, Shen L4.
Biochem Biophys Res Commun. 2015 Oct 1. pii: S0006-291X(15)30626-4. doi: 10.1016/j.bbrc.2015.09.109. [Epub ahead of print]
Author : Shi F1, Shang L1, Pan BQ1, Wang XM1, Jiang YY1, Hao JJ1, Zhang Y1, Cai Y1, Xu X1, Zhan QM1, Wang MR2.
Clin Cancer Res. 2014 Dec 1;20(23):6153-62. doi: 10.1158/1078-0432.CCR-14-0583. Epub 2014 Sep 17.
Provided below are standard protocols that you may find useful for product applications.
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene.
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