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IGF1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - IGF1 Antibody (C-term) AP14099b
    IGF1 Antibody (C-term) (Cat. #AP14099b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the IGF1 antibody detected the IGF1 protein (arrow).
  • 3 - IGF1 Antibody (C-term) AP14099b
    Confocal immunofluorescent analysis of IGF1 Antibody (C-term)(Cat#AP14099b) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • 4 - IGF1 Antibody (C-term) AP14099b
    IGF1 Antibody (C-term) (Cat. #AP14099b) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 14 - IGF1 Antibody (C-term) AP14099b
    IGF1 Antibody (C-term) (Cat. #AP14099b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 14 - IGF1 Antibody (C-term) AP14099b
    IGF1 Antibody (C-term) (Cat. #AP14099b)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, FC, IHC-P, E
Primary Accession P05019
Other Accession P01343, NP_000609.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 21841 Da
Additional info
Gene ID 3479
Other Names IGF1; IBP1; Insulin-like growth factor I; Mechano growth factor; Somatomedin-C
Target/Specificity This IGF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-144 amino acids from the C-terminal region of human IGF1.
Dilution WB~~1:1000
IF~~1:10~50
FC~~1:10~50
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIGF1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IGF1
Synonyms IBP1
Function The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (PubMed:21076856, PubMed:24132240). Ca(2+)-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb (By similarity). Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down- stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:19578119, PubMed:22351760, PubMed:23696648, PubMed:23243309).
Cellular Location Secreted {ECO:0000250|UniProtKB:P05017}.
Research Areas

BACKGROUND

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.

REFERENCES

Li, M., et al. J. Biol. Chem. 285(40):30480-30488(2010)
Canzian, F., et al. Hum. Mol. Genet. 19(19):3873-3884(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Szczesny, G., et al. Arch Orthop Trauma Surg (2010) In press :

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