- 文献引用 : 2
|Application ||WB, E|
|Other Accession||Q3UG20, Q8NFF8, NP_891847.1, NP_061152.3|
|Calculated MW||204965 Da|
|Other Names||Histone-lysine N-methyltransferase 2E, Lysine N-methyltransferase 2E, Myeloid/lymphoid or mixed-lineage leukemia protein 5, KMT2E, MLL5|
|Target/Specificity||This MLL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-120 amino acids from the N-terminal region of human MLL5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MLL5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Associates with chromatin regions downstream of transcriptional start sites of active genes and thus regulates gene transcription (PubMed:23629655, PubMed:24130829, PubMed:23798402). Chromatin interaction is mediated via the binding to tri-methylated histone H3 at 'Lys-4' (H3K4me3) (PubMed:24130829, PubMed:23798402). Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation (By similarity). Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages including G1/S transition, S phase progression and mitotic entry (PubMed:14718661, PubMed:18573682, PubMed:19264965, PubMed:23629655). Recruited to E2F1 responsive promoters by HCFC1 where it stimulates tri-methylation of histone H3 at 'Lys-4' and transcriptional activation and thereby facilitates G1 to S phase transition (PubMed:23629655). During myoblast differentiation, required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells (By similarity).|
|Cellular Location||Chromosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus speckle. Note=Absent from the nucleolus (PubMed:14718661). Localizes to chromosome during interphase and to centrosomes during mitosis (PubMed:23798402). Dissociation from mitotic chromosome is likely due to histone H3 phosphorylation on 'Thr-3' and 'Thr-6' (PubMed:23798402) Isoform NKp44L: Cytoplasm. Cell membrane; Peripheral membrane protein|
|Tissue Location||Widely expressed in both adult and fetal tissues (PubMed:12101424, PubMed:23958951). Highest levels of expression observed in fetal thymus and kidney and in adult hematopoietic tissues, jejunum and cerebellum (PubMed:12101424, PubMed:23958951). Isoform NKp44L: Not detected on circulating cells from healthy individuals, but is expressed on a large panel of tumor and transformed cells (PubMed:23958951)|
Author : Zhou P1,Ding X1,Wan X1,Liu L1,2,Yuan X1,Zhang W1,3,Hui X1,3,Meng G1,Xiao H1,Li B4,Zhong J1,Hou F5,Deng L6,Zhang Y7.
Nat Commun. 2018 Mar 28;9(1):1243. doi: 10.1038/s41467-018-03563-8.
Author : Zhou P1, Wang Z, Yuan X, Zhou C, Liu L, Wan X, Zhang F, Ding X, Wang C, Xiong S, Wang Z, Yuan J, Li Q, Zhang Y.
J Biol Chem. 2013 Jun 14;288(24):17532-43. doi: 10.1074/jbc.M112.439729. Epub 2013 Apr 29.
Provided below are standard protocols that you may find useful for product applications.
This gene is a member of the myeloid/lymphoid or mixed-lineage leukemia (MLL) family and encodes a protein with an N-terminal PHD zinc finger and a central SET domain. Overexpression of the protein inhibits cell cycle progression. Alternate transcriptional splice variants have been characterized. [provided by RefSeq].
Liu, J., et al. J. Biol. Chem. 285(27):20904-20914(2010)
Fujiki, R., et al. Nature 459(7245):455-459(2009)
Cheng, F., et al. Int. J. Biochem. Cell Biol. 40(11):2472-2481(2008)
Sun, X.J., et al. PLoS ONE 3 (1), E1499 (2008) :
Olsen, J.V., et al. Cell 127(3):635-648(2006)