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M Mlkl Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - M Mlkl Antibody (C-term) AP14272B
    All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilution Lane 1: mouse testis lysates Lane 2: mouse liver lysates Lane 3: NIH/3T3 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - M Mlkl Antibody (C-term) AP14272B
    All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilution Lane 1: NIH/3T3 whole cell lysates Lane 2: mouse spleen lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - M Mlkl Antibody (C-term) AP14272B
    Anti-Mlkl Antibody (C-term)at 1:1000 dilution + mouse lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - M Mlkl Antibody (C-term) AP14272B
    All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilution Lane 1: mouse lung lysates Lane 2: NIH/3T3 whole cell lysates Lane 3: mouse liver lysates Lane 4: mouse testis whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - M Mlkl Antibody (C-term) AP14272B
    Western blot analysis of lysate from mouse lung tissue lysate, using Mlkl Antibody (C-term)(Cat. #AP14272b). AP14272b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
  • 1 - M Mlkl Antibody (C-term) AP14272B
    Western blot analysis of lysate from mouse lung tissue lysate, using Mouse Mlkl Antibody (C-term)(Cat. #AP14272b). AP14272b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • 1 - M Mlkl Antibody (C-term) AP14272B
    Western blot analysis of lysate from mouse lung tissue lysate, using Mouse Mlkl Antibody (C-term)(Cat. #AP14272b). AP14272b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • 1 - M Mlkl Antibody (C-term) AP14272B
    Western blot analysis of lysate from mouse spleen tissue lysate, using Mouse Mlkl Antibody (C-term)(Cat. #AP14272b). AP14272b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • 1 - M Mlkl Antibody (C-term) AP14272B
    Western blot analysis of lysate from mouse lung tissue lysate, using Mouse Mlkl Antibody (C-term)(Cat. #AP14272b). AP14272b was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • 1 - M Mlkl Antibody (C-term) AP14272B
    Mouse Mlkl Antibody (C-term) (Cat. #AP14272b) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Mlkl antibody detected the Mlkl protein (arrow).
  • 产品详情
  • 文献引用 : 14
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q9D2Y4
Other Accession NP_083281.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54317 Da
Additional info
Gene ID 74568
Other Names Mixed lineage kinase domain-like protein, Mlkl {ECO:0000312|EMBL:AAH237551, ECO:0000312|MGI:MGI:1921818}
Target/Specificity This Mouse Mlkl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-472 amino acids from the C-terminal region of mouse Mlkl.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsM Mlkl Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Mlkl {ECO:0000312|EMBL:AAH23755.1, ECO:0000312|MGI:MGI:1921818}
Function Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process. Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. Does not have protein kinase activity (PubMed:23835476, PubMed:24012422, PubMed:24019532). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (By similarity).
Cellular Location Cytoplasm {ECO:0000250|UniProtKB:Q8NB16}. Cell membrane {ECO:0000250|UniProtKB:Q8NB16}. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction. {ECO:0000250|UniProtKB:Q8NB16}
Tissue Location Highly expressed in thymus, colon, intestine, liver, spleen and lung. Expressed at much lower level in skeletal muscle, heart and kidney. Not detected in brain
Research Areas
Kinase domain dimerization drives RIPK3-dependent necroptosis.
Author : Raju S1,Whalen DM2,Mengistu M3,Swanson C3,Quinn JG4,Taylor SS5,Webster JD6,Newton K7,Shaw AS8,3.
Sci Signal. 2018 Aug 21;11(544). pii: eaar2188. doi: 10.1126/scisignal.aar2188.
30131368
HECTD3 mediates TRAF3 polyubiquitination and type I interferon induction during bacterial infection.
Author : Li F,Li Y,Liang H,Xu T,Kong Y,Huang M,Xiao J,Chen X,Xia H,Wu Y,Zhou Z,Guo X,Hu C,Yang C,Cheng X,Chen C,Qi X.
J Clin Invest. 2018 Jun 19. pii: 120406. doi: 10.1172/JCI120406. [Epub ahead of print]
29920190
Pretreatment of Huaiqihuang extractum protects against cisplatin-induced nephrotoxicity.
Author : Guo Y1,2,Wang M1,Mou J3,Zhao Z1,Yang J1,Zhu F1,Pei G1,Zhu H1,Wang Y1,Xu G1,Zeng R4,Yao Y5.
Sci Rep. 2018 May 9;8(1):7333. doi: 10.1038/s41598-018-25610-6.
29743526
RIP kinase 1-dependent endothelial necroptosis underlies systemic inflammatory response syndrome.
Author : Zelic M1,Roderick JE1,O'Donnell JA1,Lehman J1,Lim SE1,Janardhan HP2,Trivedi CM2,Pasparakis M3,Kelliher MA1.
J Clin Invest. 2018 Apr 16. pii: 96147. doi: 10.1172/JCI96147. [Epub ahead of print]
29664014
Phenytoin inhibits necroptosis.
Author : von Mässenhausen A1,Tonnus W1,Himmerkus N2,Parmentier S1,Saleh D3,Rodriquez D4,Ousingsawat J5,Ang RL6,Weinberg JM7,Sanz AB8,Ortiz A8,Zierleyn A1,Becker JU9,Baratte B10,Desban N10,Bach S10,Schiessl IM5,Nogusa S11,Balachandran S11,Anders HJ12,Ting AT6,Bleic
Cell Death Dis. 2018 Mar 2;9(3):359. doi: 10.1038/s41419-018-0394-3.
29500402
Generation and Use of Chimeric RIP Kinase Molecules to Study Necroptosis.
Author : Rodriguez DA1,Green DR2.
Methods Mol Biol. 2018;1857:71-83. doi: 10.1007/978-1-4939-8754-2_7.
30136231
Embryonic Lethality and Host Immunity of RelA-Deficient Mice Are Mediated by Both Apoptosis and Necroptosis.
Author : Xu C1,Wu X1,Zhang X1,Xie Q1,2,Fan C1,Zhang H3.
J Immunol. 2017 Nov 22. pii: ji1700859. doi: 10.4049/jimmunol.1700859. [Epub ahead of print]
29167229
RIPK1-RIPK3-MLKL-dependent necrosis promotes the aging of mouse male reproductive system.
Author : Li D1,Meng L1,Xu T1,Su Y1,Liu X1,Zhang Z1,Wang X1.
Elife. 2017 Aug 15;6. pii: e27692. doi: 10.7554/eLife.27692.
28807105
Nucleotide-binding oligomerization domain (NOD) signaling defects and cell death susceptibility cannot be uncoupled in X-linked inhibitor of apoptosis (XIAP)-driven inflammatory disease.
Author : Chirieleison SM1,Marsh RA2,Kumar P1,Rathkey JK1,Dubyak GR1,Abbott DW3.
J Biol Chem. 2017 Apr 12. pii: jbc.M117.781500. doi: 10.1074/jbc.M117.781500. [Epub ahead of print]
28404814
Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling.
Author : Kang YJ1, Bang BR1, Han KH2, Hong L1, Shim EJ1, Ma J1, Lerner RA2, Otsuka M3.
Nat Commun. 2015 Sep 18;6:8371. doi: 10.1038/ncomms9371.
26381214
Necroptosis is preceded by nuclear translocation of the signaling proteins that induce it.
Author : Yoon S1, Bogdanov K1, Kovalenko A1, Wallach D1.
Cell Death Differ. 2015 Jul 17. doi: 10.1038/cdd.2015.92. [Epub ahead of print]
26184911
Characterization of RIPK3-mediated phosphorylation of the activation loop of MLKL during necroptosis.
Author : Rodriguez DA1, Weinlich R1, Brown S1, Guy C1, Fitzgerald P1, Dillon CP1, Oberst A2, Quarato G1, Low J3, Cripps JG4, Chen T3, Green DR<
Cell Death Differ. 2015 May 29. doi: 10.1038/cdd.2015.70. [Epub ahead of print]
26024392
RIP1 suppresses innate immune necrotic as well as apoptotic cell death during mammalian parturition.
Author : Kaiser WJ1, Daley-Bauer LP2, Thapa RJ3, Mandal P2, Berger SB4, Huang C2, Sundararajan A2, Guo H2, Roback L2, Speck SH2, Bertin J4, Gou
Proc Natl Acad Sci U S A. 2014 May 27;111(21):7753-8. doi: 10.1073/pnas.1401857111. Epub 2014 May 12.
24821786
Toll-like receptor 3-mediated necrosis via TRIF, RIP3, and MLKL.
Author : Kaiser WJ1, Sridharan H, Huang C, Mandal P, Upton JW, Gough PJ, Sehon CA, Marquis RW, Bertin J, Mocarski ES.
J Biol Chem. 2013 Oct 25;288(43):31268-79. doi: 10.1074/jbc.M113.462341. Epub 2013 Sep 9.
24019532

BACKGROUND

The protein kinase domain is predicted to be catalytically inactive. Molecular function: protein binding. There are two isoforms.

REFERENCES

Bisson, N., et al. Cell Cycle 7(7):909-916(2008)

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