注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   代谢   >   POLG Antibody (C-term)   

POLG Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - POLG Antibody (C-term) AP14948B
    Anti-POLG Antibody (C-term) at 1:500 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 140 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - POLG Antibody (C-term) AP14948B
    POLG Antibody (C-term) (Cat. #AP14948b) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the POLG antibody detected the POLG protein (arrow).
  • 4 - POLG Antibody (C-term) AP14948B
    POLG Antibody (C-term) (Cat. #AP14948b) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 3 - POLG Antibody (C-term) AP14948B
    Confocal immunofluorescent analysis of POLG Antibody (C-term)(Cat#AP14948b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • 14 - POLG Antibody (C-term) AP14948B
    POLG Antibody (C-term) (Cat. #AP14948b)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of POLG Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, IF, IHC-P, E
Primary Accession P54098
Other Accession Q9QYV8, P54099, NP_001119603.1, NP_002684.1
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 5428
Other Names DNA polymerase subunit gamma-1, Mitochondrial DNA polymerase catalytic subunit, PolG-alpha, POLG, MDP1, POLG1, POLGA
Target/Specificity This POLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1120-1148 amino acids from the C-terminal region of human POLG.
Dilution WB~~1:1000
FC~~1:10~50
IF~~1:10~50
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPOLG Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POLG
Synonyms MDP1, POLG1, POLGA
Function Involved in the replication of mitochondrial DNA. Associates with mitochondrial DNA.
Cellular Location Mitochondrion. Mitochondrion matrix, mitochondrion nucleoid
Research Areas

BACKGROUND

Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene.

REFERENCES

Tong, Z.B., et al. Fertil. Steril. 94(7):2932-2934(2010)
Stewart, J.D., et al. Hepatology 52(5):1791-1796(2010)
Batabyal, D., et al. J. Biol. Chem. 285(44):34191-34201(2010)
Wang, W., et al. Nucleic Acids Res. (2010) In press :
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)

FeedBack

终于等到您。ABCEPTA(百远生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-88856768 tech-china@abcepta.com.


我要评价