|Application ||IHC-P, IHC-P-Leica, WB, E|
|Other Names||Asialoglycoprotein receptor 1, ASGP-R 1, ASGPR 1, C-type lectin domain family 4 member H1, Hepatic lectin H1, HL-1, ASGR1, CLEC4H1|
|Target/Specificity||This ASGR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ASGR1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ASGR1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.|
|Cellular Location||Isoform H1a: Membrane; Single-pass type II membrane protein|
|Tissue Location||Expressed exclusively in hepatic parenchymal cells|
Provided below are standard protocols that you may find useful for product applications.
Partially deglycosylated plasma glycoproteins and immunoglobulin IgA2 allotypes are efficiently and specifically removed from circulation by a receptor-mediated process. The asialoglycoprotein receptor binds to desialylated (galactosyl-terminal) glycoproteins. It transports these glycoproteins via a series of membrane vesicles and tubules to an acidic-sorting organelle where the receptor and ligand dissociate. Then the receptor is recycled back to the cell surface and the ligand is transported to the lysosomes for degradation. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
Yang, J., et al. Arch. Virol. 155(6):881-888(2010)
Liu, J., et al. PLoS ONE 5 (9), E12934 (2010) :
Sorensen, A.L., et al. Blood 114(8):1645-1654(2009)
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Yik, J.H., et al. J. Biol. Chem. 277(43):40844-40852(2002)