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>   首页   >   产品   >   一抗   >   细胞生物学   >   TSC22D3 Antibody (Center)   

TSC22D3 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 59 - TSC22D3 Antibody (Center) AP16682c
    Immunohistochemical analysis of paraffin-embedded Human kidney tissue using AP16682c performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 59 - TSC22D3 Antibody (Center) AP16682c
    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using AP16682c performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 1 - TSC22D3 Antibody (Center) AP16682c
    All lanes : Anti-TSC22D3 Antibody (Center) at 1:2000 dilution Lane 1: Human skeletal muscle tissue lysate Lane 2: Raji whole cell lysate Lane 3: Daudi whole cell lysate Lane 4: Human lung tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 15 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P-Leica, WB, E
Primary Accession Q99576
Other Accession Q9EQZ1, P80220, NP_004080.2, NP_001015881.1
Reactivity Human, Mouse
Predicted Rat, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 1831
Other Names TSC22 domain family protein 3, DSIP-immunoreactive peptide, Protein DIP, hDIP, Delta sleep-inducing peptide immunoreactor, Glucocorticoid-induced leucine zipper protein, GILZ, TSC-22-like protein, TSC-22-related protein, TSC-22R, TSC22D3, DSIPI, GILZ
Target/Specificity This TSC22D3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the Central region of human TSC22D3.
Dilution IHC-P-Leica~~1:500
WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTSC22D3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TSC22D3
Synonyms DSIPI, GILZ
Function Protects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation. In vitro, suppresses AP1 and NFKB1 DNA-binding activities (By similarity). Isoform 1 inhibits myogenic differentiation and mediates anti- myogenic effects of glucocorticoids by binding and regulating MYOD1 and HDAC1 transcriptional activity resulting in reduced expression of MYOG (By similarity).
Cellular Location Isoform 1: Cytoplasm. Nucleus. Note=Localization depends on differentiation status of myoblasts. In undifferentiated myoblasts, isoform 1 localizes to the cytoplasm, but in differentiating myoblasts, isoform 1 is localized to the nucleus (By similarity).
Tissue Location Expressed in brain, lung, spleen and skeletal muscle. Lower levels detected in heart and kidney. Not detected in the pancreas. In non-lymphoid tissues, in the absence of inflammation, the major source of constitutive expression is the macrophage lineage. Also expressed in cells from different hemopoietic cell lineages, including bone marrow cells, CD34+ stem cells, mature B- and T-cells, monocytes and granulocytes. Down- regulated in activated macrophages from inflammatory lesions of delayed-type hypersensitivity (DTH) reactions, such as in tuberculosis and in Crohn disease, whereas in Burkitt lymphoma, persists in macrophages involved in the phagocytosis of apoptotic malignant cells.
Research Areas

BACKGROUND

The protein encoded by this gene shares significant sequence identity with the murine TSC-22 and Drosophila shs, both of which are leucine zipper proteins, that function as transcriptional regulators. The expression of this gene is stimulated by glucocorticoids and interleukin 10, and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid and chemokine. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq].

REFERENCES

Latre de Late, P., et al. J. Biol. Chem. 285(8):5594-5605(2010)
Lekva, T., et al. J. Clin. Endocrinol. Metab. 95(1):246-255(2010)
Soundararajan, R., et al. Proc. Natl. Acad. Sci. U.S.A. 106(19):7804-7809(2009)
Zhang, X.H., et al. Clin. Exp. Allergy 39(5):647-654(2009)
Redjimi, N., et al. Mol. Cancer 8, 83 (2009) :

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