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>   首页   >   产品   >   一抗   >   精选抗体   >   细胞自噬抗体   >   LC3 Antibody (APG8C) (N-term)   

LC3 Antibody (APG8C) (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - LC3 Antibody (APG8C) (N-term) AP1804a
    All lanes : Anti-APG8c (MAP1LC3C)-M1 at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: HT-1080 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 17 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - LC3 Antibody (APG8C) (N-term) AP1804a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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  • 文献引用 : 4
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession Q9BXW4
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 16852 Da
Additional info
Gene ID 440738
Other Names Microtubule-associated proteins 1A/1B light chain 3C, Autophagy-related protein LC3 C, Autophagy-related ubiquitin-like modifier LC3 C, MAP1 light chain 3-like protein 3, MAP1A/MAP1B light chain 3 C, MAP1A/MAP1B LC3 C, Microtubule-associated protein 1 light chain 3 gamma, MAP1LC3C
Target/Specificity This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLC3 Antibody (APG8C) (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP1LC3C
Function Ubiquitin-like modifier that plays a crucial role in antibacterial autophagy (xenophagy) through the selective binding of CALCOCO2. Recruits all ATG8 family members to infecting bacteria such as S.Typhimurium (PubMed:23022382). May also play a role in aggrephagy, the macroautophagic degradation of ubiquitinated and aggregated proteins (PubMed:28404643).
Cellular Location Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome Note=LC3-II binds to the autophagic membranes
Tissue Location Most abundant in placenta, lung and ovary.
Research Areas
Down-regulation of autophagy-related protein 5 (ATG5) contributes to the pathogenesis of early-stage cutaneous melanoma.
Author : Liu H1, He Z, von Rütte T, Yousefi S, Hunger RE, Simon HU.
Sci Transl Med. 2013 Sep 11;5(202):202ra123. doi: 10.1126/scitranslmed.3005864.
24027027
Triterpenes from Ganoderma Lucidum induce autophagy in colon cancer through the inhibition of p38 mitogen-activated kinase (p38 MAPK).
Author : Thyagarajan A, Jedinak A, Nguyen H, Terry C, Baldridge LA, Jiang J, Sliva D.
Nutr Cancer. 2010;62(5):630-40. doi: 10.1080/01635580903532390.
20574924
A novel mTOR activating protein protects dopamine neurons against oxidative stress by repressing autophagy related cell death.
Author : Choi KC, Kim SH, Ha JY, Kim ST, Son JH.
J Neurochem. 2010 Jan;112(2):366-76. doi: 10.1111/j.1471-4159.2009.06463.x. Epub 2009 Oct 29.
19878437
Allopregnanolone treatment delays cholesterol accumulation and reduces autophagic/lysosomal dysfunction and inflammation in Npc1-/- mouse brain.
Author : Liao G, Cheung S, Galeano J, Ji AX, Qin Q, Bi X.
Brain Res. 2009 May 13;1270:140-51. doi: 10.1016/j.brainres.2009.03.027. Epub 2009 Mar 25.
19328188

BACKGROUND

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3c is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

REFERENCES

Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
Greenberg JT. Dev Cell. 8(6):799-801. (2005)
Levine B. Cell. 120(2):159-62. (2005)
Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
Tanida I., et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004)
He H., et al. J. Biol. Chem. 278:29278-29287(2003)
Tanida I., et al. J. Biol. Chem. 279:36268-36276(2004)

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