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ATG5 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 3 - ATG5 Antibody (C-term) AP1812b
    Fluorescent image of U251 cells stained with ATG5 Antibody (C-term) (Cat#AP1812b). AP1812b was diluted at 1:25 dilution. U251 cells were treated with Chloroquine (50 μM, 16h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).
  • 1 - ATG5 Antibody (C-term) AP1812b
    Western blot analysis of APG5L Pab in Y79 cell line, mouse liver tissue, and Hela cell line lysates(Cat. #AP1812b).
  • 1 - ATG5 Antibody (C-term) AP1812b
    Cos7, HEK293, MEF, and Hela cells left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.
  • 14 - ATG5 Antibody (C-term) AP1812b
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 1 - ATG5 Antibody (C-term) AP1812b
    Western blot analysis of APG5L (arrow) using rabbit polyclonal APG5L Antibody (P224) (Cat. #AP1812b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, E
Primary Accession Q9H1Y0
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 32447 Da
Additional info
Gene ID 9474
Other Names Autophagy protein 5, APG5-like, Apoptosis-specific protein, ATG5, APG5L, ASP
Target/Specificity This ATG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-238 amino acids from the C-terminal region of human ATG5.
Dilution IF~~1:25
WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATG5 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG5
Synonyms APG5L, ASP
Function Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.
Cellular Location Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein Note=Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme
Tissue Location Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells
Research Areas
DOWNREGULATED APOPTOSIS AND AUTOPHAGY AFTER ANTI-Aβ IMMUNOTHERAPY IN ALZHEIMER'S DISEASE.
Author : Paquet C1,2,3,Nicoll JA4,5,Love S6,Mouton-Liger F2,7,Holmes C4,8,Hugon J1,2,3,Boche D4.
Brain Pathol. 2017 Oct 13. doi: 10.1111/bpa.12567. [Epub ahead of print]
29027727
Sphingosine Kinase 1 Protects Renal Tubular epithelial cells from Renal Fibrosis via Induction of Autophagy.
Author : Du C1,Ren Y1,Yao F1,Duan J2,Zhao H3,Du Y1,Xiao X1,Duan H4,Shi Y5.
Int J Biochem Cell Biol. 2017 Jul 18. pii: S1357-2725(17)30175-9. doi: 10.1016/j.biocel.2017.07.011. [Epub ahead of print]
28733250
Studying Autophagy in Zebrafish.
Author : Mathai BJ1,Meijer AH2,Simonsen A3.
Cells. 2017 Jul 9;6(3). pii: E21. doi: 10.3390/cells6030021.
28698482
Calpain-mediated cleavage of collapsin response mediator protein-2 drives acute axonal degeneration.
Author : Zhang JN1,Michel U1,Lenz C2,3,Friedel CC4,Köster S5,6,d'Hedouville Z1,Tönges L1,7,Urlaub H2,3,Bähr M1,6,Lingor P1,6,Koch JC1,6.
Sci Rep. 2016 Nov 15;6:37050. doi: 10.1038/srep37050.
27845394
The germline-enriched Ppp1r36 promotes autophagy.
Author : Zhang Q1, Gao M2, Zhang Y2, Song Y1, Cheng H1, Zhou R2.
Sci Rep. 2016 Apr 21;6:24609. doi: 10.1038/srep24609.
27098880
Promotion of Pro-Apoptotic Signals by Lysosomal Photodamage: Mechanistic Aspects and Influence of Autophagy.
Author : Kessel D1, Evans CL2.
Photochem Photobiol. 2016 Apr 20. doi: 10.1111/php.12592. [Epub ahead of print]
27096545
High glucose environment inhibits cranial neural crest survival by activating excessive autophagy in the chick embryo.
Author : Wang XY1,2, Li S1, Wang G1, Ma ZL1, Chuai M3, Cao L4, Yang X1.
Sci Rep. 2015 Dec 16;5:18321. doi: 10.1038/srep18321.
26671447
Cell type-dependent ROS and mitophagy response leads to apoptosis or necroptosis in neuroblastoma.
Author : Radogna F1, Cerella C1, Gaigneaux A1, Christov C2, Dicato M1, Diederich M3.
Oncogene. 2015 Dec 7. doi: 10.1038/onc.2015.455. [Epub ahead of print]
26640148
GMI, an immunomodulatory protein from Ganoderma microsporum, potentiates cisplatin-induced apoptosis via autophagy in lung cancer cells.
Author : Hsin IL, Ou CC, Wu MF, Jan MS, Hsiao YM, Lin CH, Ko JL.
Mol Pharm. 2015 Mar 26. [Epub ahead of print]
25811903
Early and sustained activation of autophagy in degenerating axons after spinal cord injury.
Author : Ribas VT1, Schnepf B, Challagundla M, Koch JC, Bähr M, Lingor P.
Brain Pathol. 2015 Mar;25(2):157-70. doi: 10.1111/bpa.12170. Epub 2014 Sep 12.
25040536
Autophagy in zebrafish.
Author : Varga M1, Fodor E1, Vellai T2.
Methods. 2014 Dec 9. pii: S1046-2023(14)00397-1. doi: 10.1016/j.ymeth.2014.12.004. [Epub ahead of print]
25498006
Coxsackievirus B3 induces crosstalk between autophagy and apoptosis to benefit its release after replicating in autophagosomes through a mechanism involving caspase cleavage of autophagy-related proteins.
Author : Xin L1, Xiao Z1, Ma X1, He F1, Yao H1, Liu Z2.
Infect Genet Evol. 2014 May 15;26C:95-102. doi: 10.1016/j.meegid.2014.05.005. [Epub ahead of print]
24836289
Circadian and noncircadian modulation of autophagy in photoreceptors and retinal pigment epithelium.
Author : Yao J1, Jia L1, Shelby SJ1, Ganios AM2, Feathers K1, Thompson DA2, Zacks DN1.
Invest Ophthalmol Vis Sci. 2014 Apr 29;55(5):3237-46. doi: 10.1167/iovs.13-13336.
24781939
Autophagy restricts Chlamydia trachomatis growth in human macrophages via IFNG-inducible guanylate binding proteins.
Author : Al-Zeer MA, Al-Younes HM, Lauster D, Abu Lubad M, Meyer TF.
Autophagy. 2013 Jan;9(1):50-62. doi: 10.4161/auto.22482. Epub 2012 Oct 19.
23086406
Induction of autophagy is essential for monocyte-macrophage differentiation.
Author : Zhang Y, Morgan MJ, Chen K, Choksi S, Liu ZG.
Blood. 2012 Mar 22;119(12):2895-905. doi: 10.1182/blood-2011-08-372383. Epub 2012 Jan 5.
22223827
Arsenic trioxide enhances the radiation sensitivity of androgen-dependent and -independent human prostate cancer cells.
Author : Chiu HW, Chen YA, Ho SY, Wang YJ.
PLoS One. 2012;7(2):e31579. doi: 10.1371/journal.pone.0031579. Epub 2012 Feb 20.
22363680
Expression pattern and functions of autophagy-related gene atg5 in zebrafish organogenesis.
Author : Hu Z, Zhang J, Zhang Q.
Autophagy. 2011 Dec;7(12):1514-27.
22082871
Critical role for hyperpolarization-activated cyclic nucleotide-gated channel 2 in the AIF-mediated apoptosis.
Author : Norberg E, Karlsson M, Korenovska O, Szydlowski S, Silberberg G, Uhlén P, Orrenius S, Zhivotovsky B.
EMBO J. 2010 Nov 17;29(22):3869-78. doi: 10.1038/emboj.2010.253. Epub 2010 Oct 29.
21037554
The class IA phosphatidylinositol 3-kinase p110-beta subunit is a positive regulator of autophagy.
Author : Dou Z, Chattopadhyay M, Pan JA, Guerriero JL, Jiang YP, Ballou LM, Yue Z, Lin RZ, Zong WX.
J Cell Biol. 2010 Nov 15;191(4):827-43. doi: 10.1083/jcb.201006056. Epub 2010 Nov 8.
21059846
Therapeutic potential of a synthetic lethal interaction between the MYC proto-oncogene and inhibition of aurora-B kinase.
Author : Yang D, Liu H, Goga A, Kim S, Yuneva M, Bishop JM.
Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13836-41. doi: 10.1073/pnas.1008366107. Epub 2010 Jul 19.
20643922
Immunohistochemical evidence for macroautophagy in neurones and endothelial cells in Alzheimer's disease.
Author : Ma JF, Huang Y, Chen SD, Halliday G.
Neuropathol Appl Neurobiol. 2010 Jun;36(4):312-9. doi: 10.1111/j.1365-2990.2010.01067.x. Epub 2010 Jan 21.
20102518
Oxidative modification sensitizes mitochondrial apoptosis-inducing factor to calpain-mediated processing.
Author : Norberg E, Gogvadze V, Vakifahmetoglu H, Orrenius S, Zhivotovsky B.
Free Radic Biol Med. 2010 Mar 15;48(6):791-7. doi: 10.1016/j.freeradbiomed.2009.12.020. Epub 2010 Jan 4.
20043986
Biochemical isolation and characterization of the tubulovesicular LC3-positive autophagosomal compartment.
Author : Gao W, Kang JH, Liao Y, Ding WX, Gambotto AA, Watkins SC, Liu YJ, Stolz DB, Yin XM.
J Biol Chem. 2010 Jan 8;285(2):1371-83. doi: 10.1074/jbc.M109.054197. Epub 2009 Nov 12.
19910472
Control of basal autophagy by calpain1 mediated cleavage of ATG5.
Author : Xia HG, Zhang L, Chen G, Zhang T, Liu J, Jin M, Ma X, Ma D, Yuan J.
Autophagy. 2010 Jan;6(1):61-6. Epub 2010 Jan 13.
19901552
Lysosomal-associated protein multispanning transmembrane 5 gene (LAPTM5) is associated with spontaneous regression of neuroblastomas.
Author : Inoue J, Misawa A, Tanaka Y, Ichinose S, Sugino Y, Hosoi H, Sugimoto T, Imoto I, Inazawa J.
PLoS One. 2009 Sep 29;4(9):e7099. doi: 10.1371/journal.pone.0007099.
19787053
A novel protein complex in membrane rafts linking the NR2B glutamate receptor and autophagy is disrupted following traumatic brain injury.
Author : Bigford GE, Alonso OF, Dietrich D, Keane RW.
J Neurotrauma. 2009 May;26(5):703-20. doi: 10.1089/neu.2008.0783.
19335206
Association of autophagy defect with a malignant phenotype and poor prognosis of hepatocellular carcinoma.
Author : Ding ZB, Shi YH, Zhou J, Qiu SJ, Xu Y, Dai Z, Shi GM, Wang XY, Ke AW, Wu B, Fan J.
Cancer Res. 2008 Nov 15;68(22):9167-75. doi: 10.1158/0008-5472.CAN-08-1573.
19010888

BACKGROUND

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

REFERENCES

Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) Greenberg JT. Dev Cell. 8(6):799-801. (2005) Levine B. Cell. 120(2):159-62. (2005) Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004) Hammond E.M., et al. FEBS Lett. 425:391-395(1998) Strausberg R.L., et al. PNAS 99:16899-16903(2002) Grand R.J.A., et al. Exp. Cell Res. 218:439-451(1995) Mizushima N., et al. J. Biol. Chem. 273:33889-33892(1998) Mizushima N., et al. J. Cell Biol. 152:657-668(2001)

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