- 文献引用 : 2
|Application ||IHC-P, WB, E|
|Calculated MW||24636 Da|
|Other Names||Regulator of G-protein signaling 19, RGS19, G-alpha-interacting protein, GAIP, RGS19, GAIP, GNAI3IP|
|Target/Specificity||This RGS19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of human RGS19.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RGS19 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G-alpha subfamily 1 members, with the order G(i)a3 > G(i)a1 > G(o)a >> G(z)a/G(i)a2. Activity on G(z)-alpha is inhibited by phosphorylation and palmitoylation of the G-protein.|
|Cellular Location||Membrane; Lipid-anchor.|
|Tissue Location||Highest expression in lung. Placenta, liver and heart also express high levels of GAIP|
Author : Li X1, Wang D1, Chen Z1, Lu E1, Wang Z1, Duan J1, Tian W1, Wang Y1, You L1, Zou Y1, Cheng Y1, Zhu Q2, Wan X3, Xi
Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):4731-6. doi: 10.1073/pnas.1503779112. Epub 2015 Mar 30.
Author : Sanchez CG1, Penfornis P, Oskowitz AZ, Boonjindasup AG, Cai DZ, Dhule SS, Rowan BG, Kelekar A, Krause DS, Pochampally RR.
Carcinogenesis. 2011 Jul;32(7):964-72. doi: 10.1093/carcin/bgr029. Epub 2011 Feb 11.
Provided below are standard protocols that you may find useful for product applications.
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). RGS19 enhances the intrinsic GTPase-activating protein activity of the Galphai3 protein, which stimulates autophagy by favoring the GDP-bound form of Galphai3.
Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
Greenberg JT. Dev Cell. 8(6):799-801. (2005)
Levine B. Cell. 120(2):159-62. (2005)
Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
de Vries L., et al. PNAS 93:15203-15208(1996)
de Alba E., et al. J. Mol. Biol. 291:927-939(1999)
Wang J., et al. J. Biol. Chem. 273:26014-26025(1998)
Ogier-Denis E., et al. J. Biol. Chem. 275:39090-39095(2000)