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RGS19 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RGS19 Antibody (N-term) AP1820a
    All lanes : Anti-RGS19-H5 at 1:2000 dilution Lane 1: HT-29 whole cell lysate Lane 2: Human lung tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - RGS19 Antibody (N-term) AP1820a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P49795
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 24636 Da
Additional info
Gene ID 10287
Other Names Regulator of G-protein signaling 19, RGS19, G-alpha-interacting protein, GAIP, RGS19, GAIP, GNAI3IP
Target/Specificity This RGS19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of human RGS19.
Dilution WB~~1:2000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRGS19 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RGS19
Synonyms GAIP, GNAI3IP
Function Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G-alpha subfamily 1 members, with the order G(i)a3 > G(i)a1 > G(o)a >> G(z)a/G(i)a2. Activity on G(z)-alpha is inhibited by phosphorylation and palmitoylation of the G-protein.
Cellular Location Membrane; Lipid-anchor.
Tissue Location Highest expression in lung. Placenta, liver and heart also express high levels of GAIP
Research Areas
Gαi1 and Gαi3 regulate macrophage polarization by forming a complex containing CD14 and Gab1.
Author : Li X1, Wang D1, Chen Z1, Lu E1, Wang Z1, Duan J1, Tian W1, Wang Y1, You L1, Zou Y1, Cheng Y1, Zhu Q2, Wan X3, Xi
Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):4731-6. doi: 10.1073/pnas.1503779112. Epub 2015 Mar 30.
25825741
Activation of autophagy in mesenchymal stem cells provides tumor stromal support.
Author : Sanchez CG1, Penfornis P, Oskowitz AZ, Boonjindasup AG, Cai DZ, Dhule SS, Rowan BG, Kelekar A, Krause DS, Pochampally RR.
Carcinogenesis. 2011 Jul;32(7):964-72. doi: 10.1093/carcin/bgr029. Epub 2011 Feb 11.
21317300

BACKGROUND

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). RGS19 enhances the intrinsic GTPase-activating protein activity of the Galphai3 protein, which stimulates autophagy by favoring the GDP-bound form of Galphai3.

REFERENCES

Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
Greenberg JT. Dev Cell. 8(6):799-801. (2005)
Levine B. Cell. 120(2):159-62. (2005)
Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
de Vries L., et al. PNAS 93:15203-15208(1996)
de Alba E., et al. J. Mol. Biol. 291:927-939(1999)
Wang J., et al. J. Biol. Chem. 273:26014-26025(1998)
Ogier-Denis E., et al. J. Biol. Chem. 275:39090-39095(2000)

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