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RNF145 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RNF145 Antibody (C-term) AP18281B
    Anti-RNF145 Antibody (C-term) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 76 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - RNF145 Antibody (C-term) AP18281B
    Anti-RNF145 Antibody (C-term) at 1:2000 dilution + U-87MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 76 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q96MT1
Other Accession NP_653327.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 75617 Da
Additional info
Gene ID 153830
Other Names RING finger protein 145, RNF145
Target/Specificity This RNF145 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 601-627 amino acids from the C-terminal region of human RNF145.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRNF145 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNF145 {ECO:0000312|PROSITE:PS50089}
Function E3 ubiquitin ligase that catalyzes the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. In response to bacterial infection, negatively regulates the phagocyte oxidative burst by controlling the turnover of the NADPH oxidase complex subunits. Promotes monoubiquitination of CYBA and 'Lys-48'-linked polyubiquitination and degradation of CYBB NADPH oxidase catalytic subunits, both essential for the generation of antimicrobial reactive oxygen species. Involved in the maintenance of cholesterol homeostasis. In response to high sterol concentrations ubiquitinates HMGCR, a rate-limiting enzyme in cholesterol biosynthesis, and targets it for degradation. The interaction with INSIG1 is required for this function. In addition, triggers ubiquitination of SCAP, likely inhibiting its transport to the Golgi apparatus and the subsequent processing/maturation of SREBPF2, ultimately downregulating cholesterol biosynthesis.
Cellular Location Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q5SWK7}; Multi-pass membrane protein
Research Areas
Identification of the ER-resident E3 ubiquitin ligase RNF145 as a novel LXR-regulated gene.
Author : Cook EC1,Nelson JK1,Sorrentino V1,2,Koenis D1,Moeton M1,Scheij S1,Ottenhoff R1,Bleijlevens B1,Loregger A1,Zelcer N1.
PLoS One. 2017 Feb 23;12(2):e0172721. doi: 10.1371/journal.pone.0172721. eCollection 2017.
28231341

BACKGROUND

The function of this protein remains unknown.

REFERENCES

Soranzo, N., et al. Nat. Genet. 41(11):1182-1190(2009)

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