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EIF4B Antibody (S422)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - EIF4B Antibody (S422) AP1950d
    Western blot analysis of EIF4B (arrow) using rabbit polyclonal EIF4B Antibody (S422) (RB13724). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4B gene (Lane 2) (Origene Technologies).
  • 14 - EIF4B Antibody (S422) AP1950d
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EIF4B-S422, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P23588
Other Accession Q8WYK5
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 69151 Da
Additional info
Gene ID 1975
Other Names Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B
Target/Specificity This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-429 amino acids from human EIF4B.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF4B Antibody (S422) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF4B
Function Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Research Areas

BACKGROUND

EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. It also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F

REFERENCES

Doepker, R.C., et al., J. Virol. 78(9):4684-4699 (2004).
Fleming, K., et al., Biochemistry 42(30):8966-8975 (2003).
Milburn, S.C., et al., EMBO J. 9(9):2783-2790 (1990).

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