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>   首页   >   产品   >   一抗   >   信号转导   >   Importin alpha-3 (KPNA4)Antibody (C-term)   

Importin alpha-3 (KPNA4)Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Importin alpha-3 (KPNA4)Antibody (C-term) AP1968a
    The anti-Importin alpha-4 Pab (Cat. #AP1968a) is used in Western blot to detect Importin alpha-4 in Hela (left), mouse brain (middle), and HepG2 (right)cell line/tissue lysates.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession O00629
Other Accession O35343
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 3840
Other Names Importin subunit alpha-3, Importin alpha Q1, Qip1, Karyopherin subunit alpha-4, KPNA4, QIP1
Target/Specificity This Importin alpha-4 (KPNA4) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the C-terminal region of human Importin alpha-4 (KPNA4).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsImportin alpha-3 (KPNA4)Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KPNA4
Synonyms QIP1
Function Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
Cellular Location Cytoplasm. Nucleus
Tissue Location Highly expressed in testis, ovary, small intestine, heart, skeletal muscle, lung and pancreas, but barely detectable in kidney, thymus, colon and peripheral blood leukocytes
Research Areas

BACKGROUND

The nuclear import of karyophilic proteins is directed by short amino acid sequences termed nuclear localization signals (NLSs). Karyopherins, or importins, are cytoplasmic proteins that recognize NLSs and dock NLS-containing proteins to the nuclear pore complex. The protein encoded by this gene shares the sequence similarity with Xenopus importin-alpha and Saccharomyces cerevisiae Srp1. This protein is found to interact with the NLSs of DNA helicase Q1 and SV40 T antigen.

REFERENCES

Dvorin, J.D., et al., J. Virol. 76(23):12087-12096 (2002).
Limon, A., et al., J. Virol. 76(21):10598-10607 (2002).
Hariton-Gazal, E., et al., Biochim. Biophys. Acta 1594(2):234-242 (2002).
Depienne, C., et al., J. Biol. Chem. 276(21):18102-18107 (2001).
Bouyac-Bertoia, M., et al., Mol. Cell 7(5):1025-1035 (2001).

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