注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   精选抗体   >   G蛋白偶联受体抗体(GPCR)   >   ADRA1D Antibody (N-term)   

ADRA1D Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - ADRA1D Antibody (N-term) AP20589a
    Overlay histogram showing MCF-7 cells stained with AP20589a(green line). The cells were fixed with 2% paraformaldehyde 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP20589a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 14 - ADRA1D Antibody (N-term) AP20589a
    Immunohistochemical analysis of paraffin-embedded H. kidney section using ADRA1D Antibody (N-term)(Cat#AP20589a). AP20589a was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - ADRA1D Antibody (N-term) AP20589a
    Immunohistochemical analysis of paraffin-embedded H. small intestine section using ADRA1D Antibody (N-term)(Cat#AP20589a). AP20589a was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - ADRA1D Antibody (N-term) AP20589a
    Immunohistochemical analysis of paraffin-embedded R. kidney section using ADRA1D Antibody (N-term)(Cat#AP20589a). AP20589a was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 1 - ADRA1D Antibody (N-term) AP20589a
    Western blot analysis of lysates from A549, LNCaP, PC-3 cell line and rat brain tissue lysate (from left to right), using ADRA1D Antibody (N-term) (Cat. #AP20589a). AP20589a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P25100
Reactivity Human, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 60463 Da
Additional info
Gene ID 146
Other Names Alpha-1D adrenergic receptor, Alpha-1A adrenergic receptor, Alpha-1D adrenoreceptor, Alpha-1D adrenoceptor, Alpha-adrenergic receptor 1a, ADRA1D, ADRA1A
Target/Specificity This ADRA1D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-30amino acids from the N-terminal region of human ADRA1D.
Dilution FC~~1:25
IHC-P~~1:25
WB~~1:1000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADRA1D Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADRA1D
Synonyms ADRA1A
Function This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.
Cellular Location Cell membrane; Multi-pass membrane protein.
Research Areas

BACKGROUND

This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.

REFERENCES

Bruno J.F.,et al.Biochem. Biophys. Res. Commun. 179:1485-1490(1991).
Forray C.,et al.Mol. Pharmacol. 45:703-708(1994).
Schwinn D.A.,et al.J. Pharmacol. Exp. Ther. 272:134-142(1995).
Weinberg D.H.,et al.Biochem. Biophys. Res. Commun. 201:1296-1304(1994).
Esbenshade T.A.,et al.Mol. Pharmacol. 47:977-985(1995).

FeedBack

终于等到您。ABCEPTA(百远生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-88856768 tech-china@abcepta.com.


我要评价