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CDKN1A Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - CDKN1A Antibody (C-term) AP20643c
    AP20643C staining CDKN1A in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - CDKN1A Antibody (C-term) AP20643c
    All lanes : Anti-CDKN1A Antibody (C-term) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - CDKN1A Antibody (C-term) AP20643c
    Fluorescent image of A549 cells stained with CDKN1A Antibody (C-term)(Cat#AP20643c). AP20643c was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red). Cytoplasm
  • 1 - CDKN1A Antibody (C-term) AP20643c
    Western blot analysis of lysates from Hela, HUVEC, MCF-7, SH-SY5Y cell line and mouse lung tissue lysate(from left to right), using CDKN1A Antibody (C-term)(Cat. #AP20643c). AP20643c was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • 1 - CDKN1A Antibody (C-term) AP20643c
    Western blot analysis of lysate from Hela cell line, using CDKN1A Antibody (C-term)(Cat. #AP20797c). AP20797c was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P, E
Primary Accession P38936
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 18119 Da
Additional info
Gene ID 1026
Other Names Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1
Target/Specificity This CDKN1A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 133-164 amino acids from the C-terminal region of human CDKN1A.
Dilution IHC-P~~1:25
WB~~1:1000
IF~~1:25
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCDKN1A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDKN1A
Synonyms CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WA
Function May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739).
Cellular Location Cytoplasm. Nucleus.
Tissue Location Expressed in all adult tissues, with 5-fold lower levels observed in the brain
Research Areas

BACKGROUND

May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin- dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D- CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.

REFERENCES

Harper J.W.,et al.Cell 75:805-816(1993).
El-Deiry W.S.,et al.Cell 75:817-825(1993).
Xiong Y.,et al.Nature 366:701-704(1993).
Jiang H.,et al.Mol. Cell. Differ. 1:285-299(1993).
Jiang H.,et al.Oncogene 10:1855-1864(1995).

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