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GNG3 Antibody (C-Term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 4 - GNG3 Antibody (C-Term) AP22128b
    Overlay histogram showing U-87 MG cells stained with AP22128b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22128b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - GNG3 Antibody (C-Term) AP22128b
    All lanes : Anti-GNG3 Antibody (C-Term) at 1:2000 dilution Lane 1: human fetal brain lysate Lane 2: mouse brain lysate Lane 3: human brain lysate Lane 4: mouse cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 8 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession P63215
Other Accession P63214, P63216
Reactivity Human, Mouse
Predicted Bovine, Mouse
Host Rabbit
Clonality polyclonal
Isotype Rabbit Ig
Calculated MW 8305 Da
Additional info
Gene ID 2785
Other Names Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-3, GNG3, GNGT3
Target/Specificity This GNG3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 39-69 amino acids from human GNG3.
Dilution FC~~1:25
WB~~1:2000
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGNG3 Antibody (C-Term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GNG3
Synonyms GNGT3
Function Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.
Cellular Location Cell membrane; Lipid-anchor; Cytoplasmic side
Research Areas

BACKGROUND

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction.

REFERENCES

Peng Y.,et al.Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
Hurowitz E.H.,et al.DNA Res. 7:111-120(2000).
Ding J.B.,et al.Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
Puhl H.L. III,et al.Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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