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ADD1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - ADD1 Antibody (Center) AP22310c
    Overlay histogram showing A431 cells stained with AP22310c(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - ADD1 Antibody (Center) AP22310c
    All lanes : Anti-ADD1 Antibody (Center) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: U-2 OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 81 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, FC, E
Primary Accession P35611
Other Accession Q5RA10
Reactivity Human, Rat
Host Rabbit
Clonality polyclonal
Isotype Rabbit IgG
Calculated MW 80955 Da
Additional Information
Gene ID 118
Other Names Alpha-adducin, Erythrocyte adducin subunit alpha, ADD1, ADDA
Target/Specificity This ADD1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 428-462 amino acids from the Central region of human ADD1.
Dilution WB~~1:2000
FC~~1:25
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADD1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADD1
Synonyms ADDA
Function Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Cellular Location Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side
Tissue Location Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

REFERENCES

Joshi R.L.,et al.J. Cell Biol. 115:665-675(1991).
Goldberg Y.P.,et al.Hum. Mol. Genet. 1:669-675(1992).
Lin B.,et al.Genomics 25:93-99(1995).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Hillier L.W.,et al.Nature 434:724-731(2005).

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