癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
POLA1 Antibody (N-Term)
Purified Rabbit Polyclonal Antibody (Pab)
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- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | P09884 |
| Other Accession | P33609, O89042 |
| Reactivity | Human |
| Predicted | Mouse, Rat |
| Host | Rabbit |
| Clonality | polyclonal |
| Isotype | Rabbit Ig |
| Calculated MW | 165913 Da |
| Gene ID | 5422 |
|---|---|
| Other Names | DNA polymerase alpha catalytic subunit, 2.7.7.7, DNA polymerase alpha catalytic subunit p180, POLA1, POLA |
| Target/Specificity | This POLA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-33 amino acids from the human region of human POLA1. |
| Dilution | WB~~1:2000 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | POLA1 Antibody (N-Term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | POLA1 |
|---|---|
| Synonyms | POLA |
| Function | Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA:DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses (PubMed:27019227). |
| Cellular Location | Nucleus. Cytoplasm, cytosol. Note=In the cytosol, colocalizes with RNA:DNA hybrids with a speckled pattern |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.
REFERENCES
Wong S.W.,et al.EMBO J. 7:37-47(1988).
Pearson B.E.,et al.Mol. Cell. Biol. 11:2081-2095(1991).
Hsi K.-L.,et al.Nucleic Acids Res. 18:6231-6237(1990).
Smale S.T.,et al.Mol. Cell. Biol. 6:4077-4087(1986).
Lee S.S.,et al.Proc. Natl. Acad. Sci. U.S.A. 92:7882-7886(1995).
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