|Application ||WB, FC, E|
|Calculated MW||57580 Da|
|Other Names||DnaJ homolog subfamily C member 3, Endoplasmic reticulum DNA J domain-containing protein 6, ER-resident protein ERdj6, ERdj6, Interferon-induced, double-stranded RNA-activated protein kinase inhibitor, Protein kinase inhibitor of 58 kDa, Protein kinase inhibitor p58, DNAJC3, P58IPK, PRKRI|
|Target/Specificity||This DNAJC3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 189-223 amino acids from the Central region of human DNAJC3.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DNAJC3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions (By similarity). Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.|
|Cellular Location||Endoplasmic reticulum.|
|Tissue Location||Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.|
Provided below are standard protocols that you may find useful for product applications.
Involved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
Korth M.J.,et al.Gene 170:181-188(1996).
Dunham A.,et al.Nature 428:522-528(2004).
Polyak S.J.,et al.J. Biol. Chem. 271:1702-1707(1996).
Gale M.J. Jr.,et al.Mol. Cell. Biol. 18:859-871(1998).
Melville M.W.,et al.J. Biol. Chem. 274:3797-3803(1999).