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LGG-1/Atg8 antibodies

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - LGG-1/Atg8 antibodies AP22381b
    For each well, 30 worms were picked into Laemmli buffer and boiled at 95℃ for 15 min. Lysates were then used for SDS PAGE/western blotting. All antibodies (including 3 new ones to be tested for Abgent) were diluted 1:1000 in 1% milk in TBST. #83 was used as control (original Abgent #7952).DA2123 is a transgenic C. elegans strain that expresses GFP-tagged LGG-1 protein (Kang et al., Genes & Develop, 2007);#97 RB59577, #98 RB59578, and #99 RB59579 can recognize LGG-1 and GFP::LGG-1, albeit not as potently as the original Abgent antibody.(Data courtesy of Dr. Yongzhi Yang, Hansen lab SBP Institute, July 2018.)
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q09490
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 14764 Da
Additional info
Other Names Protein lgg-1, lgg-1
Target/Specificity This lgg-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 20-55 amino acids from the center region of CAEEL lgg-1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsLGG-1/Atg8 antibodies is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
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