- 文献引用 : 6
|Application ||WB, IF, E|
|Other Names||Glycogen debranching enzyme, Glycogen debrancher, 4-alpha-glucanotransferase, Oligo-1, 4-1, 4-glucantransferase, Amylo-alpha-1, 6-glucosidase, Amylo-1, 6-glucosidase, Dextrin 6-alpha-D-glucosidase, AGL, GDE|
|Target/Specificity||This AGL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 357-387 amino acids from the Central region of human AGL.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||AGL Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Multifunctional enzyme acting as 1,4-alpha-D-glucan:1,4- alpha-D-glucan 4-alpha-D-glycosyltransferase and amylo-1,6- glucosidase in glycogen degradation.|
|Cellular Location||Cytoplasm. Note=Under glycogenolytic conditions localizes to the nucleus|
|Tissue Location||Liver, kidney and lymphoblastoid cells express predominantly isoform 1; whereas muscle and heart express not only isoform 1, but also muscle-specific isoform mRNAs (isoforms 2, 3 and 4). Isoforms 5 and 6 are present in both liver and muscle|
Author : Guin S1, Ru Y2, Agarwal N3, Ritterson Lew C4, Owens C2, Comi GP5, Theodorescu D6.
Clin Cancer Res. 2015 Oct 21. pii: clincanres.1706.2015. [Epub ahead of print]
Author : Irimia JM1, Tagliabracci VS2, Meyer CM1, Segvich DM1, DePaoli-Roach AA1, Roach PJ3.
J Biol Chem. 2015 Jul 27. pii: jbc.M115.673897. [Epub ahead of print]
Author : DePaoli-Roach AA1, Tagliabracci VS, Segvich DM, Meyer CM, Irimia JM, Roach PJ.
J Biol Chem. 2010 Aug 13;285(33):25372-81. doi: 10.1074/jbc.M110.148668. Epub 2010 Jun 10.
Author : Parker KC, Kong SW, Walsh RJ; Bch, Salajegheh M, Moghadaszadeh B, Amato AA, Nazareno R, Lin YY, Krastins B, Sarracino DA, Beggs AH, Pinkus JL, Greenberg SA.
Muscle Nerve. 2009 Jun;39(6):739-53. doi: 10.1002/mus.21230.
Author : Tagliabracci VS, Girard JM, Segvich D, Meyer C, Turnbull J, Zhao X, Minassian BA, Depaoli-Roach AA, Roach PJ.
J Biol Chem. 2008 Dec 5;283(49):33816-25. doi: 10.1074/jbc.M807428200. Epub 2008 Oct 13.
Author : Cheng A1, Zhang M, Gentry MS, Worby CA, Dixon JE, Saltiel AR.
Genes Dev. 2007 Oct 1;21(19):2399-409.
Provided below are standard protocols that you may find useful for product applications.
AGL is a glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in the AGL gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing.
Horinishi, A., et al., J. Hum. Genet. 47(2):55-59 (2002).
Bao, Y., et al., Genomics 38(2):155-165 (1996).
Yang, B.Z., et al., J. Biol. Chem. 267(13):9294-9299 (1992).
Yang-Feng, T.L., et al., Genomics 13(4):931-934 (1992).
Bao, Y., et al., Gene 197 (1-2), 389-398 (1997).