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AKR1A1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - AKR1A1 Antibody (C-term) AP2734b
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AKR1A1 antibody (C-term) (Cat.#AP2734b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 1 - AKR1A1 Antibody (C-term) AP2734b
    Western blot analysis of anti-AKR1A1 Antibody (C-term) (Cat.#AP2734b) in Y79 cell line lysates (35ug/lane). AKR1A1(arrow) was detected using the purified Pab.
  • 1 - AKR1A1 Antibody (C-term) AP2734b
    Western blot analysis of AKR1A1 (arrow) using rabbit polyclonal AKR1A1 Antibody (C-term) (Cat.#AP2734b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKR1A1 gene.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P14550
Other Accession P51635, P50578, Q9JII6, Q3ZCJ2, Q6IAZ4
Reactivity Human
Predicted Bovine, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 36573 Da
Additional info
Gene ID 10327
Other Names Alcohol dehydrogenase [NADP(+)], Aldehyde reductase, Aldo-keto reductase family 1 member A1, AKR1A1, ALDR1, ALR
Target/Specificity This AKR1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 293-325 amino acids from the C-terminal region of human AKR1A1.
Dilution IHC-P~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAKR1A1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AKR1A1
Synonyms ALDR1, ALR
Function Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides and bile acids, with a preference for negatively charged substrates, such as glucuronate and succinic semialdehyde (PubMed:10510318). Functions as a detoxifiying enzyme by reducing a range of toxic aldehydes. Reduces methylglyoxal and 3- deoxyglucosone, which are present at elevated levels under hyperglycemic conditions and are cytotoxic. Involved also in the detoxification of lipid-derived aldehydes like acrolein (By similarity). Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN) (PubMed:18276838, PubMed:11306097). Displays no reductase activity towards retinoids (By similarity).
Cellular Location Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9JII6}. Apical cell membrane {ECO:0000250|UniProtKB:Q9JII6}
Tissue Location Widely expressed. Highly expressed in kidney, salivary gland and liver. Detected in trachea, stomach, brain, lung, prostate, placenta, mammary gland, small intestine and lung
Research Areas

BACKGROUND

AKR1A1 is a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue.

REFERENCES

Steuber,H.,J. Mol. Biol. 379 (5), 991-1016 (2008)
Bohren,K.M.,Biochim. Biophys. Acta 1748 (2), 201-212 (2005)
El-Kabbani,O.,Acta Crystallogr. D Biol. Crystallogr. 50 (PT 6), 859-868 (1994)

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