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Phospho-P21CIP1(T145) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Phospho-P21CIP1(T145) Antibody AP3189a
    The anti-Phospho-P21CIP1-T145 Pab (Cat. #AP3189a) is used in Western blot to detect Phospho-P21CIP1-T145 in Hela tissue lysate.
  • 6 - Phospho-P21CIP1(T145) Antibody AP3189a
    Dot blot analysis of anti-Phospho-P21CIP1-T145 Antibody (Cat. #AP3189a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.
  • 14 - Phospho-P21CIP1(T145) Antibody AP3189a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, DB, IHC-P, E
Primary Accession P38936
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 18119 Da
Additional info
Gene ID 1026
Other Names Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, MDA-6, p21, CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1
Target/Specificity This P21CIP1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T145 of human P21CIP1.
Dilution WB~~1:1000
DB~~1:500
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein A affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-P21CIP1(T145) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CDKN1A
Synonyms CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WA
Function May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding (PubMed:11595739).
Cellular Location Cytoplasm. Nucleus.
Tissue Location Expressed in all adult tissues, with 5-fold lower levels observed in the brain
Research Areas
Anticancer Activity of γ-Bisabolene in Human Neuroblastoma Cells via Induction of p53-Mediated Mitochondrial Apoptosis.
Author : Jou YJ1, Hua CH2, Lin CS3, Wang CY4, Wan L5, Lin YJ6, Huang SH7, Lin CW8,9.
Molecules. 2016 May 7;21(5). pii: E601. doi: 10.3390/molecules21050601.
27164076
Specific function of phosphoinositide 3-kinase beta in the control of DNA replication.
Author : Marqués M1, Kumar A, Poveda AM, Zuluaga S, Hernández C, Jackson S, Pasero P, Carrera AC.
Proc Natl Acad Sci U S A. 2009 May 5;106(18):7525-30. doi: 10.1073/pnas.0812000106. Epub 2009 Apr 22.
19416922

BACKGROUND

P21CIP1 is a potent cyclin-dependent kinase inhibitor. This protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. Expression is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. P21CIP1 can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein has been reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.

REFERENCES

Scott, S.A., et al., Leuk. Res. 28(12):1293-1301 (2004).
Amini, S., et al., J. Biol. Chem. 279(44):46046-46056 (2004).
Chen, T., et al., Cancer Res. 64(20):7412-7419 (2004).
Sieburg, M., et al., J. Virol. 78(19):10399-10409 (2004).
Giraud, S., et al., Oncogene 23(44):7391-7398 (2004).

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