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Phospho-STAT3(Y705) Antibody

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - Phospho-STAT3(Y705) Antibody AP3261a
    Western blot analysis of extracts from Jurkat and Hela cells, untreated or treated with IFN-α(100ng/ml), using Phospho-STAT3-pY705 Antibody.
  • 产品详情
  • 文献引用 : 6
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  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P40763
Other Accession P52631, Q19S50, P42227, P61635
Reactivity Human
Predicted Mouse, Rat, Pig, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 6774
Other Names Signal transducer and activator of transcription 3, Acute-phase response factor, STAT3, APRF
Target/Specificity This STAT3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y705 of human STAT3.
Dilution WB~~1:500
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-STAT3(Y705) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name STAT3 (HGNC:11364)
Function Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors (PubMed:10688651, PubMed:12359225, PubMed:12873986, PubMed:15194700, PubMed:17344214, PubMed:18242580, PubMed:23084476). Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene (PubMed:17344214). May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:12873986). Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes (PubMed:12359225). Activated by IL31 through IL31RA (PubMed:15194700). Acts as a regulator of inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity (PubMed:28065600). Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1 (PubMed:17344214). Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation (By similarity). May play an apoptotic role by transctivating BIRC5 expression under LEP activation (PubMed:18242580). Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity (PubMed:23084476). Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion (By similarity).
Cellular Location Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm. Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3. Identified in a complex with LYN and PAG1
Tissue Location Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas
Research Areas
SOCS3 overexpression inhibits advanced glycation end product-induced EMT in proximal tubule epithelial cells.
Author : Yu L1,2,3,Zhang Y4,Zhang H1,2,3,Li Y1,2,3.
Exp Ther Med. 2017 Jun;13(6):3109-3115. doi: 10.3892/etm.2017.4297. Epub 2017 Apr 4.
28587386
Evodiamine Induces Apoptosis and Inhibits Migration of HCT-116 Human Colorectal Cancer Cells.
Author : Zhao LC1,2, Li J3, Liao K4, Luo N5, Shi QQ6, Feng ZQ7, Chen DL8.
Int J Mol Sci. 2015 Nov 16;16(11):27411-27421.
26580615
Reg3g Promotes Pancreatic Carcinogenesis in a Murine Model of Chronic Pancreatitis.
Author : Yin G1, Du J, Cao H, Liu X, Xu Q, Xiang M.
Dig Dis Sci. 2015 Jul 17. [Epub ahead of print]
26182900
Expression of signal transducer and activator of transcription 3 and its phosphorylated form is significantly upregulated in patients with papillary thyroid cancer.
Author : Yan LI1, Li LI2, Li Q1, DI W1, Shen W1, Zhang L1, Guo H1.
Exp Ther Med. 2015 Jun;9(6):2195-2201. Epub 2015 Apr 7.
26136959
Effect of suppressor of cytokine signaling on hepcidin production in hepatitis C virus replicon cells.
Author : Miyachi H, Kobayashi Y, Relja B, Fujita N, Iwasa M, Gabazza EC, Takei Y.
Hepatol Res. 2011 Apr;41(4):364-74. doi: 10.1111/j.1872-034X.2011.00777.x. Epub 2011 Feb 23.
21348906
Cooperation between integrin alpha5 and tetraspan TM4SF5 regulates VEGF-mediated angiogenic activity.
Author : Choi S, Lee SA, Kwak TK, Kim HJ, Lee MJ, Ye SK, Kim SH, Kim S, Lee JW.
Blood. 2009 Feb 19;113(8):1845-55. doi: 10.1182/blood-2008-05-160671. Epub 2008 Nov 25.
19036703

BACKGROUND

STAT3 is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein.

REFERENCES

Schick, N., et al., J. Biol. Chem. 279(37):38787-38796 (2004).
Wang, H., et al., Lab. Invest. 84(8):941-951 (2004).
Kato, T., et al., J. Biol. Chem. 279(30):31076-31080 (2004).
Ivanova, A.V., et al., J. Mol. Biol. 340(4):641-653 (2004).
Park, J., et al., Biochem. Biophys. Res. Commun. 320(1):279-285 (2004).

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