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CD80 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CD80 Antibody (C-term) AP5020b
    All lanes : Anti-CD80 Antibody (C-term) at 1:500-1000 dilution Lane 1: Raji whole cell lysate Lane 2: CCRF-CEM whole cell lysate Lane 3: Human spleen tissue lysate Lane 4: Daudi whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession P33681
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Other Names T-lymphocyte activation antigen CD80, Activation B7-1 antigen, BB1, CTLA-4 counter-receptor B71, B7, CD80, CD80, CD28LG, CD28LG1, LAB7
Target/Specificity This CD80 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-288 amino acids from the C-terminal region of human CD80.
Dilution WB~~1:500-1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCD80 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CD80
Synonyms CD28LG, CD28LG1, LAB7
Function Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed on activated B-cells, macrophages and dendritic cells EMBL; M27533; AAA36045.1; -; mRNA EMBL; M83077; AAA58390.1; -; Genomic_DNA EMBL; M83072; AAA58390.1; JOINED; Genomic_DNA EMBL; M83073; AAA58390.1; JOINED; Genomic_DNA EMBL; M83074; AAA58390.1; JOINED; Genomic_DNA EMBL; AY197777; AAO39208.1; -; mRNA EMBL; AY197778; AAO39209.1; -; mRNA EMBL; AC073352; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC042665; AAH42665.1; -; mRNA CCDS; CCDS2989.1; -. [P33681-1] PIR; I54495; A45803 RefSeq; NP_005182.1; NM_005191.3. [P33681-1] UniGene; Hs.838; - PDB; 1DR9; X-ray; 3.00 A; A=35-233 PDB; 1I8L; X-ray; 3.00 A; A/B=35-242 PDBsum; 1DR9; - PDBsum; 1I8L; - ProteinModelPortal; P33681; - SMR; P33681; - BioGrid; 107379; 75 DIP; DIP-6044N; - IntAct; P33681; 6 STRING; 9606.ENSP00000264246; - ChEMBL; CHEMBL2364157; - DrugBank; DB01281; Abatacept DrugBank; DB06681; Belatacept DrugBank; DB04901; Galiximab GuidetoPHARMACOLOGY; 2744; - iPTMnet; P33681; - PhosphoSitePlus; P33681; - BioMuta; CD80; - DMDM; 461606; - PaxDb; P33681; - PeptideAtlas; P33681; - PRIDE; P33681; - ProteomicsDB; 54922; - Ensembl; ENST00000264246; ENSP00000264246; ENSG00000121594. [P33681-1] Ensembl; ENST00000383669; ENSP00000373165; ENSG00000121594. [P33681-2] Ensembl; ENST00000478182; ENSP00000418364; ENSG00000121594. [P33681-1] GeneID; 941; - KEGG; hsa:941; - UCSC; uc003ecq.4; human. [P33681-1] CTD; 941; - DisGeNET; 941; - EuPathDB; HostDB:ENSG00000121594.11; - GeneCards; CD80; - HGNC; HGNC:1700; CD80 HPA; CAB025368; - HPA; HPA050092; - MIM; 112203; gene neXtProt; NX_P33681; - OpenTargets; ENSG00000121594; - PharmGKB; PA26239; - eggNOG; ENOG410J3XE; Eukaryota eggNOG; ENOG411154Z; LUCA GeneTree; ENSGT00940000162632; - HOGENOM; HOG000036959; - HOVERGEN; HBG055207; - InParanoid; P33681; - KO; K05412; - OMA; VRIYWQK; - OrthoDB; 349094at2759; - PhylomeDB; P33681; - TreeFam; TF351094; - Reactome; R-HSA-1257604; PIP3 activates AKT signaling Reactome; R-HSA-2219530; Constitutive Signaling by Aberrant PI3K in Cancer Reactome; R-HSA-389356; CD28 co-stimulation Reactome; R-HSA-389357; CD28 dependent PI3K/Akt signaling Reactome; R-HSA-389359; CD28 dependent Vav1 pathway Reactome; R-HSA-389513; CTLA4 inhibitory signaling Reactome; R-HSA-6783783; Interleukin-10 signaling Reactome; R-HSA-6811558; PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling SIGNOR; P33681; - EvolutionaryTrace; P33681; - GeneWiki; CD80; - GenomeRNAi; 941; - PRO; PR:P33681; - Proteomes; UP000005640; Chromosome 3 Bgee; ENSG00000121594; Expressed in 74 organ(s), highest expression level in gastrocnemius Genevisible; P33681; HS GO; GO:0009986; C:cell surface; HDA:UniProtKB GO; GO:0009897; C:external side of plasma membrane; IBA:GO_Central GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW GO; GO:0005886; C:plasma membrane; TAS:Reactome GO; GO:0098636; C:protein complex involved in cell adhesion; IDA:MGI GO; GO:0015026; F:coreceptor activity; NAS:UniProtKB GO; GO:0046934; F:phosphatidylinositol-4,5-bisphosphate 3-kinase activity; TAS:Reactome GO; GO:0001618; F:virus receptor activity; IEA:UniProtKB-KW GO; GO:0007166; P:cell surface receptor signaling pathway; IBA:GO_Central GO; GO:0071222; P:cellular response to lipopolysaccharide; IBA:GO_Central GO; GO:0019221; P:cytokine-mediated signaling pathway; TAS:Reactome GO; GO:0006955; P:immune response; IBA:GO_Central GO; GO:0035556; P:intracellular signal transduction; NAS:UniProtKB GO; GO:0042130; P:negative regulation of T cell proliferation; IBA:GO_Central GO; GO:0045425; P:positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; NAS:UniProtKB GO; GO:0045086; P:positive regulation of interleukin-2 biosynthetic process; NAS:UniProtKB GO; GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; IDA:UniProtKB GO; GO:0051897; P:positive regulation of protein kinase B signaling; TAS:Reactome GO; GO:0009967; P:positive regulation of signal transduction; NAS:UniProtKB GO; GO:0042102; P:positive regulation of T cell proliferation; IBA:GO_Central GO; GO:0045627; P:positive regulation of T-helper 1 cell differentiation; NAS:UniProtKB GO; GO:0045893; P:positive regulation of transcription, DNA-templated; NAS:UniProtKB GO; GO:0007165; P:signal transduction; IBA:GO_Central GO; GO:0031295; P:T cell costimulation; IBA:GO_Central CDD; cd16083; IgC_CD80; 1 Gene3D; 2.60.40.10; -; 2 InterPro; IPR013162; CD80_C2-set InterPro; IPR037676; CD80_IgC InterPro; IPR007110; Ig-like_dom InterPro; IPR036179; Ig-like_dom_sf InterPro; IPR013783; Ig-like_fold InterPro; IPR003599; Ig_sub InterPro; IPR013106; Ig_V-set Pfam; PF08205; C2-set_2; 1 Pfam; PF07686; V-set; 1 SMART; SM00409; IG; 1 SUPFAM; SSF48726; SSF48726; 2 PROSITE; PS50835; IG_LIKE; 2 1: Evidence at protein level; 3D-structure; Alternative splicing; Complete proteome; Direct protein sequencing; Disulfide bond; Glycoprotein; Host cell receptor for virus entry; Host-virus interaction; Immunoglobulin domain; Membrane; Phosphoprotein; Receptor; Reference proteome; Signal; Transmembrane; Transmembrane helix SIGNAL 1 34 CHAIN 35 288 T-lymphocyte activation antigen CD80 /FTId=PRO_0000014547 TOPO_DOM 35 242 Extracellular. TRANSMEM 243 263 Helical. TOPO_DOM 264 288 Cytoplasmic. DOMAIN 35 135 Ig-like V-type DOMAIN 145 230 Ig-like C2-type MOD_RES 284 284 Phosphoserine CARBOHYD 53 53 N-linked (GlcNAc...) asparagine CARBOHYD 89 89 N-linked (GlcNAc...) asparagine CARBOHYD 98 98 N-linked (GlcNAc...) asparagine CARBOHYD 186 186 N-linked (GlcNAc...) asparagine CARBOHYD 207 207 N-linked (GlcNAc...) asparagine CARBOHYD 211 211 N-linked (GlcNAc...) asparagine CARBOHYD 226 226 N-linked (GlcNAc...) asparagine CARBOHYD 232 232 N-linked (GlcNAc...) asparagine DISULFID 50 116 {ECO:0000255|PROSITE-ProRule:PRU00114, ECO:0000269|PubMed:11279502} DISULFID 162 216 {ECO:0000255|PROSITE-ProRule:PRU00114, ECO:0000269|PubMed:11279502} VAR_SEQ 140 140 A -> G (in isoform 3) /FTId=VSP_047698 VAR_SEQ 141 266 Missing (in isoform 3) /FTId=VSP_047699 VAR_SEQ 234 266 TKQEHFPDNLLPSWAITLISVNGIFVICCLTYC -> S (in isoform 2) /FTId=VSP_047700 STRAND 37 41 {ECO:0000244|PDB:1DR9} STRAND 46 48 {ECO:0000244|PDB:1DR9} HELIX 58 61 {ECO:0000244|PDB:1DR9} STRAND 63 68 {ECO:0000244|PDB:1DR9} STRAND 71 77 {ECO:0000244|PDB:1DR9} STRAND 80 83 {ECO:0000244|PDB:1DR9} HELIX 85 88 {ECO:0000244|PDB:1DR9} STRAND 91 95 {ECO:0000244|PDB:1DR9} TURN 96 99 {ECO:0000244|PDB:1DR9} STRAND 100 103 {ECO:0000244|PDB:1DR9} HELIX 108 110 {ECO:0000244|PDB:1DR9} STRAND 112 120 {ECO:0000244|PDB:1DR9} STRAND 127 139 {ECO:0000244|PDB:1DR9} STRAND 146 151 {ECO:0000244|PDB:1DR9} STRAND 157 169 {ECO:0000244|PDB:1DR9} STRAND 171 179 {ECO:0000244|PDB:1DR9} STRAND 185 191 {ECO:0000244|PDB:1DR9} TURN 193 195 {ECO:0000244|PDB:1DR9} STRAND 198 207 {ECO:0000244|PDB:1DR9} STRAND 212 220 {ECO:0000244|PDB:1DR9} STRAND 225 231 {ECO:0000244|PDB:1DR9} SEQUENCE 288 AA; 33048 MW; BA453EE34528B1F4 CRC64; MGHTRRQGTS PSKCPYLNFF QLLVLAGLSH FCSGVIHVTK EVKEVATLSC GHNVSVEELA QTRIYWQKEK KMVLTMMSGD MNIWPEYKNR TIFDITNNLS IVILALRPSD EGTYECVVLK YEKDAFKREH LAEVTLSVKA DFPTPSISDF EIPTSNIRRI ICSTSGGFPE PHLSWLENGE ELNAINTTVS QDPETELYAV SSKLDFNMTT NHSFMCLIKY GHLRVNQTFN WNTTKQEHFP DNLLPSWAIT LISVNGIFVI CCLTYCFAPR CRERRRNERL RRESVRPV
Research Areas

BACKGROUND

CD80 is activation antigen B7-1 (formerly referred to as B7) provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 (MIM 186760) and CTLA4 (MIM 123890) ligands of T cells.

REFERENCES

Mosbruger, T.L., et al. J. Infect. Dis. 201(9):1371-1380(2010)
Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)
Segat, L., et al. J. Gastroenterol. Hepatol. 24(12):1840-1846(2009)

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